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Kinetik der Dihydrofolsäurereduktase aus Rattenleber und -gehirn mit 3-APADPH+H+ als Wasserstoffdonator

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Summary

An enzyme preparation capable to reduce dihydrofolic acid to tetrahydrofolic acid, was prepared from liver and brain of rats. Using a spectrophotometric method the reaction rates of these preparations were determined. For rat liver dihydrofolic acid reductase K m was found to 2×10−6 M for NADPH + H+ and 1.8×10−6 M for FH2. Rat brain dihydrofolic acid-reductase has a K m of 8×10−6 M for NADPH + H+. Folic acid was shown to be a competitive inhibitor of the conversion of dihydrofolate to tetrahydrofolate. With 3-APADPH + H+ which is formed in brain of rats after application of 3-acetylpyridine, the enzyme activity of both preparations was found to be significantly decreased. These observations are discussed in relation to the functional impairment of rats treated with 3-acetylpyridine.

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Authors and Affiliations

  1. Aus dem Pharmakologischen Institut der Freien Universität Berlin, Germany

    H. Herken & R. Timmler

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  1. H. Herken
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  2. R. Timmler
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Mit 4 Textabbildungen

Ein Teil der Ergebnisse wurde auf der 5. Frühjahrstagung der Deutschen Pharmakologischen Gesellschaft vorgetragen.

Mit Unterstützung durch die Deutsche Forschungsgemeinschaft.

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Herken, H., Timmler, R. Kinetik der Dihydrofolsäurereduktase aus Rattenleber und -gehirn mit 3-APADPH+H+ als Wasserstoffdonator. Naunyn - Schmiedebergs Arch 250, 293–301 (1965). https://doi.org/10.1007/BF00258522

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  • DOI: https://doi.org/10.1007/BF00258522

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