Summary
The effect of high glucose concentrations on continuous ethanol production by passively immobilized Zymomonas mobilis cells has been studied. High effluent ethanol concentrations always led to low productivities. The maximum ethanol concentration attained was 92.8 g/l (98% glucose conversion) at a dilution rate of 0.14 h-1 with 200 g/l glucose medium. The observed enhancement of cell immobilization in the fibrous support at high glucose concentrations in the feed input seems to be related to the formation of bacterial filaments.
References
Amin G, Verachte H (1982) Comparative study of ethanol production by immobilized-cell system using Zymomonas mobilis or Saccharomyces bayanus. Eur J Appl Microbiol Biotechnol 14:59–63
Amin G, Eynde E van den, Verachtert H (1983) Determination of by-products formed during the ethanolic fermentation, using batch and immobilized cell system of Zymomonas mobilis and Saccharomyces bayanus. Eur J Appl Mirobiol Biotechnol 18:1–5
Bajpai PK, Margaritis A (1985) Kinetics of ethanol production by immobilized cells of Zymomonas mobilis at varying d-glucose concentration. Enzyme Microb Technol 7:462–464
Baratti JC, Bu'Lock JD (1986) Zymomonas mobilis: a bacterium for ethanol production. Biotechnol Adv 4:95–115
Borrego F, Obón JM, Cánovas M, Manjón A, Iborra JL (1987) Effect of temperature and long-term operation on passively immobilized Zymomonas mobilis for continuous ethanol production. Biotechnol Lett 9:573–576
Borrego F, Obón JM, Cánovas M, Manjón A, Iborra JL (1988) PH influence on ethanol production and retained biomass in a passively immobilized Zymomonas mobilis system. Biotechnol Lett 10:437–442
Bringer S, Sahm H, Swyzen W (1984) Ethanol production by Zymomonas mobilis and its application on an industrial scale. Biotechnol Bioeng Symp 14:311–319
Buchholz SE, Dooley MM, Eveleigh DE (1987) Zymomonas: an alcoholic enigma. Trends Biotechnol 5:199–204
Fein JE, Lawford HG, Lawford GR, Zawadzki BC, Charley RC (1983) High productivity continuous ethanol fermentation with a flocculating mutant strain of Zymomonas mobilis. Biotechnol Lett 5:19–24
Jain WK, Toran-Díaz I, Baratti JC (1985) Continuous production of ethanol from fructose by immobilized growing cells of Zymomonas mobilis. Biotechnol Bioeng 27:613–620
Jöbses IML, Egberts GTC, Luyben KCAM, Roels JA (1986) Fermentation kinetics of Zymomonas mobilis at high ethanol concentrations: oscillations in continuous culture. Biotechnol Bioeng 28:868–877
Klein J, Kressdorf B (1986) Rapid ethanol fermentation with immobilized Zymomonas mobilis in a three stage reactor system. Biotechnol Lett 8:739–744
Lee KJ, Tribe DE, Rogers PL (1979) Ethanol production by Zymomonas mobilis in continuous culture at high glucose concentrations. Biotechnol Lett 1:421–426
Margaritis A, Merchant FJA (1984) Advances in ethanol production using immobilized cell systems. CRC Crit Rev Biotechnol 1:339–393
Montenecourt BS (1985) Zymomonas, a unique genus of bacteria. In: Demain AL, Solomon NA (eds) Biology of industrial microorganisms. Benjamin/Cummings, Menlo Park, Calif, pp 261–289
Rogers PL, Lee KJ, Skotnicki ML, Tribe DE (1982) Ethanol production by Zymomonas mobilis. In: Fiechter A (ed) Advances in Biochemical Engineering, vol 23. Springer, New York, Heidelberg, Berlin, pp 37–84
Shriner RH, Fuson RC, Curtin DY (1972) Identificación sistemática de compuestos orgánicos. Limusa-Willey, Mexico D.F. Mexico, pp 131–133
Stevnsborg N, Lawford HG, Martin N, Beveridge T (1986) Effect of growth temperature on the morphology and performance of Zymomonas mobilis ATCC 29191 in batch and continuous culture. Appl Microbiol Biotechnol 25:116–123
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Castellar, M.R., Borrego, F., Cánovas, M. et al. Continuous ethanol production at high glucose concentrations by a passively immobilized Zymomonas mobilis system. Appl Microbiol Biotechnol 31, 249–252 (1989). https://doi.org/10.1007/BF00258404
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00258404