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Continuous (R)-mandelic acid production in an enzyme membrane reactor

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Summary

The continuous conversion of phenylglyoxylic acid to (R)-mandelic acid was performed in an enzyme membrane reactor with simultaneous coenzyme regeneration employing mandelate dehydrogenase and formate dehydrogenase. A mathematical model of the coupled enzyme reactions was formulated and applied to determine optimal conditions for (R)-mandelic acid production. The experiments and calculations showed that the optimal operational points depend strongly on enzyme kinetics. The individual kinetic parameters determined were appropriate for the description of (R)-mandelic acid production in the simultaneous two-enzyme process. Space time yields of 700 g/(l·day) were obtained at low enzyme consumption.

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Abbreviations

C :

concentration (mmol/l, mM)

K i :

inhibition constants (mmol/l, mM)

K m :

apparent Michaelis-Menten constants (mmol/l, mM)

P :

productivity (g/(dm3 day))

R :

overall reaction rate (mmol/l/min)

t :

time (min)

v 0 :

initial reaction rate (units/mg)

V max :

maximal reaction rate (units/mg)

τ:

residence time (min)

References

  • Hummel W, Schütte H, Kula M-R (1988) d-(-)Mandelic acid dehydrogenase from Lactobacillus curvatus. Appl Microbiol Biotechnol 28:433–439

    Google Scholar 

  • Wandrey C, Wichmann R, Kula M-R, Bückmann AF (1984) Enzym-Membran-Reaktor. Die Umschau 3:88–91

    Google Scholar 

  • Wichmann R, Wandrey C, Bückmann AF, Kula M-R (1981) Continuous enzymatic transformation in an enzyme membrane reactor with simultaneous NAD(H) regeneration. Biotechnol Bioeng 23:2789–2802

    CAS  Google Scholar 

  • Cordes A, Kula M-R (1986) Process design for large scale purification of formate dehydrogenase from Candida boidinii affinity partition. J Chromatogr 376:375–384

    Google Scholar 

  • Bückmann AF, Morr M, Kula M-R (1987) Preparation of technical grade polyethylenglycol (PEG) (MR=20000)-N6-(2-Aminoethyl)-NADH by a procedure adaptable to large-scale synthesis. Biotechnol Appl Biochem 9:258–268

    Google Scholar 

  • Hoffmann U, Hofmann H (1971) Einführung in der Optimierung mit Anwendungsbeispielen aus dem Chemie-Ingenieur-Wesen. Verlag Chemie, Weinheim

    Google Scholar 

  • Zurmühl R (1965) Praktische Mathematik für Ingenieure und Physiker. Springer, Berlin, Heidelberg, New York

    Google Scholar 

  • O'Neill SP (1971) Steady-state stability of substrate inhibited enzyme catalysis in open reactor systems. Biotechnol Bioeng 13:493–502

    Google Scholar 

  • O'Neill SP, Lilly MD, Rowe PN (1971) Multiple steady states in continuous-flow, stirred-tank enzyme reactors. Chem Eng Sci 26:173–175

    Google Scholar 

  • Schmidt E, Fiolitaktis E, Wandrey C (1987) Multiple steady states in a coupled enzyme system represented by the enzymatically catalyzed production of l-phenylalanine, in: Enzyme Engineering 8 (Laskin AI, Mosbach K, Thomas D, Wingard LB, eds), Ann NY Acad Sci 501: 434–437

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Authors and Affiliations

  1. Faculty of Technology, University of Zagreb, Zagreb, Yugoslavia

    Dj. Vasič-Racki

  2. Institute of Biotechnology, Nuclear Research Centre, Jülich, Federal Republic of Germany

    M. Jonas & C. Wandrey

  3. Institute of Enzyme Technology, University of Düsseldorf, D-5170, Jülich, Federal Republic of Germany

    W. Hummel & M. R. Kula

Authors
  1. Dj. Vasič-Racki
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  2. M. Jonas
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  3. C. Wandrey
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  4. W. Hummel
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  5. M. R. Kula
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Vasič-Racki, D., Jonas, M., Wandrey, C. et al. Continuous (R)-mandelic acid production in an enzyme membrane reactor. Appl Microbiol Biotechnol 31, 215–222 (1989). https://doi.org/10.1007/BF00258398

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  • DOI: https://doi.org/10.1007/BF00258398

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