Summary
The water status and nitrogen metabolism of the groundnut (Arachis hypogaea L.) cultivars, M-13 and M-145 were examined during a period of water stress and recovery. 10 day old seedlings, grown in controlled environment were exposed to solutions of polyethylene glycol (MW 6000, osmotic potential, −0.5 or −0.8 MPa = −5 or −8 bars) for a week when the stress was relieved by replacing the PEG solution with a nutrient solution. Relative turgidity, total protein and free amino acid concentration and nitrate reductase activity were measured during the period of stress and recovery. Cultivar M-13, despite its lower relative turgidity during stress, was better able to preserve its protein concentration and nitrate reductase activity, and recover to a normal state within three days of the relief of stress. M-145, on the other hand, failed to recover within this period. The results are discussed in the light of the known performance of the two cultivars in the field under drought conditions.
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References
Arnon DI, Hoagland DR (1940) Crop production in artificial culture solutions and in soils with special reference to factors influencing yields and adsorption of inorganic nutrients. Soil Sci 50:463–84
Cheung YNS, Tyree MT, Dainty J (1975) Water relations parameters on single leaves obtained in a pressure bomb and some ecological implications. Can J Bot 53:1342–46
Dedio W (1975) Water relations in wheat leaves, as screening tests for drought resistance. Can J Plant Sci 55:369–78
Hageman RH, Leng ER, Dudley JW (1967) A biochemical approach to plant breeding. Advan Agron 19:45–86
Hsiao TC (1973) Plant responses to water stress. Annu Rev Plant Physiol 24:519–70
Hurd EA (1976) Plant breeding for drought resistance. In: Kozlowski TT (ed) Water deficits and plant growth, Vol IV. Soil Water Measurement, Plant Responses and Breeding for Drought Resistance. Academic Press, New York, pp 317–353
Jaworski E (1971) Nitrate reductase assay in intact plant tissue. Biochem Biophys Res Commun 43:1274–79
Lee YP, Takahashi T (1966) An improved colorimetric determination of amino acids with the use of ninhydrin. Analytical Biochem 14:71–77
Lowry OH, Rosebrough NS, Paurr AL, Randall RJ (1951) Protein measurement with Folin-Phenol reagent. J Biol Chem 193:265–75
Parmar MT, Moore RP (1968) Carbowax 6000, mannitol and sodium chloride for simulating drought conditions in germination studies of corn (Zea mays L.) of strong and weak vigour. Agron J 60:192–95
Sandhu RS, Jaswal SV, Sangha AS (1975) The performance of variety M-13 in comparison with some approved varieties of groundnut (Arachis hypogaea L.) in Punjab. J Res Punjab Agric Univ 11:350–54
Todd GW, Webster DL (1965) Effect of repeated drought period on photosynthesis and survival of cereal seedlings. Agron J 57:399–404
Weatherley PE (1950) Studies in the water relations of the cotton plant. I. The field measurement of water deficit in leaves. New Phytol 49:81–97
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Saini, H.S., Srivastava, A.K. Osmotic stress and the nitrogen metabolism of two groundnut (Arachis Hypogaea L.) cultivars. Irrig Sci 2, 185–192 (1981). https://doi.org/10.1007/BF00257980
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DOI: https://doi.org/10.1007/BF00257980