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Human prostatic reverse transcriptase and RNA-virus

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Summary

Methods for the assay of the RNA-dependent DNA polymerase which has the characteristics of the enzyme in murine leukemia virus have been adapted for study of extracts of human prostate in a search for RNA viral activity. Of the extracts from a total of 25 glands submitted to the evolved screening procedure 6 or 24% proved active enough to warrant further characterization, including tests of template dependence and specificity. In this set the extract from a carcinoma proved far more potent than any of the five coming from benign hyperplastic glands. It was not only dependent upon the presence of template but was a lot more responsive to the template most effective with virus, i. e., poly rA · oligo T (12 to 18) than with poly rA · poly T. Finally, the elution pattern of this extract's enzyme activity closely resembled that obtained from authentic viral extract. It was concluded from these preliminary experiences that this analytical scheme may be a simple means to investigate viral association with prostatic cancer.

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  1. Department of Biochemistry and Urology, Veterans Administration Hospital, Buffalo, New York, USA

    Wells E. Farnsworth

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  1. Wells E. Farnsworth
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Farnsworth, W.E. Human prostatic reverse transcriptase and RNA-virus. Urol. Res. 1, 106–112 (1973). https://doi.org/10.1007/BF00257372

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  • DOI: https://doi.org/10.1007/BF00257372

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