Summary
The uptake and metabolism of radioactive ADP, AMP and adenosine was investigated in the pulmonary circulation of lungs taken from rats with streptozotocin-induced diabetes. Between 0.26 and 500 μmol/l ADP was extensively hydrolysed to AMP equally in control and diabetic lungs. At 1 mmol/l there was less ADP breakdown in diabetic lungs. Hydrolysis of AMP to adenosine was also less in diabetic lungs at 10 μmol/l and 1 mmol/l substrate concentration, but adenosine metabolism and uptake at these concentrations was not affected by diabetes. The results indicate that formation of the anti-aggregatory adenosine and, to a lesser extent, breakdown of the pro-aggregatory ADP were decreased in diabetic lungs and may contribute to the platelet hyperreactivity associated with diabetes.
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References
Alabaster VA, Bakhle YS (1970) Removal of 5-hydroxytryptamine in the pulmonary circulation of rat isolated lungs. Br J Pharmacol 40: 468–482
Bakhle YS, Chelliah R (1983) Metabolism of adenine nucleotides and adenosine in the pulmonary circulation of isolated lungs from man and other animals. J Physiol 336: 42 P
Bern MD (1978) Platelet functions in diabetes mellitus. Diabetes 27: 342–352
Crutchley DJ, Ryan US, Ryan JW (1980) Effects of aspirin and dipyridamole on the degradation of adenosine diphosphate by cultured cells derived from bovine pulmonary artery. J Clin Invest 66: 29–35
Dieterle Y, Ody C, Ehrensberger A, Stalder H, Junod AF (1978) Metabolism and uptake of adenosine triphosphate and adenosine by porcine aortic and pulmonary endothelial cells and fibroblasts in culture. Circ Res 42: 869–876
Johnson M, Harrison HE, Hawker R, Hawker L (1979) Platelet abnormalities in experimental diabetes. Thromb Haemostasis 42: 333 (Abstract)
Lagarde M, Burtin M, Berciaud P, Blanc M, Velardo B, Dechavanne M (1980) Increase of platelet thromboxane A2 formation and of its plasmatic half-life in diabetes mellitus. Thromb Res 19: 823–830
Lieberman GE, Lewis GP, Peters TJ (1977) A membrane-bound enzyme in rabbit aorta capable of inhibiting adenosine diphosphate induced platelet aggregation. Lancet 2: 330–332
Mejbaum-Katzenellenbogen W, Dobryszycka WM (1959) Microtannin turbidity assay for protein. Clin Chim Acta 4: 513–522
Norman GA, Follet MJ, Hector DA (1974) Quantitative thin layer chromatography of ATP and its degradation products in meat tissue. J Chromatogr 90: 105–111
Pearson JD, Carleton JS, Hutchings A, Gordon JL (1978) Uptake and metabolism of adenosine by pig aortic endothelial and smooth-muscle cells in culture. Biochem J 170: 265–271
Pearson JD, Carleton JS, Gordon JL (1980) Metabolism of adenine nucleotides by ectoenzymes of vascular endothelial and smooth-muscle cells. Biochem J 190: 421–429
Shimizu H, Creveling CR, Daly J (1970) Stimulated formation of adenosine 3′5′ cyclic phosphate in cerebral cortex: synergism between electrical activity and biogenic amines. Proc Natl Acad Sci USA 65: 1035–1040
Strum JM, Junod AF (1972) Radioautographic demonstration of 5-hydroxytryptamine-3H uptake by pulmonary endothelial cells. J Cell Biol 54: 456–467
Watts IS, Zakrzewski JT, Bakhle YS (1982) Altered prostaglandin synthesis in isolated lungs of rats with streptozotocin-induced diabetes. Thromb Res 28: 333–342
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Bakhle, Y.S., Chelliah, R. Effect of streptozotocin-induced diabetes on the metabolism of ADP, AMP and adenosine in the pulmonary circulation of rat isolated lung. Diabetologia 24, 455–459 (1983). https://doi.org/10.1007/BF00257347
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DOI: https://doi.org/10.1007/BF00257347