Summary
The number of nuclear thyroxine (T4) or triiodothyronine (T3) receptors and the serum values of thyroxine, triiodothyronine, reverse triiodothyronine and TSH were investigated in 13 patients with Type 1 (insulin-dependent) diabetes (group 1), in 10 patients with Type 2 (non-insulin-dependent) diabetes (group 2) and in age and weight matched non-diabetic subjects. All patients were clinically euthyroid, although serum T3 was low and reverse T3 high in group 1 compared with the non-diabetic subjects. The Type 1 diabetic patients had evidence of poor metabolic control because of weight loss and high glycosylated haemoglobin levels. The maximal specific nuclear binding capacities for T4 (1.8×10−16mol T4/10μg DNA) and T3 (1.4×10−16mol T3/10 μg DNA) were increased in group 1 compared with the normal subjects (T4∶1.1×10−16 mol T4/10 μg DNA, p<0.010, T3∶0.9×10−16mol T3/10 μg DNA, p<0.05), whereas the nuclear binding affinity for T4 (Ka=1.5×109 l/mol) and T3 (5.1×109 l/mol) was similar to that of the normal subjects (T4, Ka=2.0×109 l/mol; T3, Ka=5.6×109 l/mol). In contrast, neither the nuclear binding of T4 (1.0×10−16mol T4/10 μg DNA) and T3 (1.1×10−16 mol T3/10 ug DNA) nor the binding affinity for T4 (Ka=3.3×109 l/mol) and T3 (Ka=3.9×109 l/mol) in group 2 differed from those of the non-diabetic subjects. In conclusion, nuclear T4 and T3 receptor number in cells from patients with poorly controlled Type 1 diabetes was raised compared with normal subjects. This increase may have been responsible for the euthyroidism in these patients in whom the serum T3 was low. In contrast, cellular binding of T4 and T3 appeared normal in the Type 2 diabetic subjects.
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References
Saunders J, Hall SEH, Sönksen PH (1978) Thyroid hormones in insulin requiring diabetes before and after treatment. Diabetologia 15: 20–32
Böyum A (1968) Separation of leucocytes from blood and bone marrow. Scand J Clin Invest 21: 77–89
Burton K (1956) The conditions and mechanisms and the Diphenylamin reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J 62: 315–322
Scatchard G (1949) The attraction of proteins for small molecules and ions. Ann N Y Acad Sci 51: 660–672
Alexander CM, Kaptein EM, Lum SMC, Spencer CA, Kumar D, Nicoloff JT (1982) Pattern of recovery of thyroid hormone indices associated with treatment of diabetes mellitus. J Clin Endocrinol Metab 54: 362–366
Fujii S, Akai T, Tanaka S, Nakatani K, Kinoshita M, Seki J, Wada M (1981) Thyroid hormone abnormalities in patients with diabetes mellitus. J Endocrinol Invest 4: 71–74
Chopra IJ, Wiersinga W, Frank H (1981) Alterations in hepatic monodeiodination of iodothyronines in the diabetic rat. Life Sci 28: 1765–1776
Vaganakis AG, Portnay GI, O'Brian JT, Rudolph M, Arky RA, Ingbar SH, Braverman LE (1977) Effects of starvation on the production and metabolism of thyroxine and triiodothyronine in euthyroid obese patients. J Clin Endocinol Metab 45: 1305–1309
Burman MD, Latham KR, Ajua YY, Smallridge RC, Tseng YL, Lukes YG, Maunder R, Wartofsky L (1980) Solubilized nuclear thyroid hormone receptors in circulating human mononuclear cells. J Clin Endocrinol Metab 51: 106–116
Wiersinga WM, Frank HJL, Chopra IJ, Solomon PH (1982) Alterations in hepatic nuclear binding of triiodothyronine in experimental diabetes mellitus in rats. Acta Endocrinol 99: 79–85
Burman KD, Lukes Y, Wright FD, Wartofsky L (1977) Reduction in hepatic triiodothyronine binding capacity induced by fasting. Endocrinology 101: 1331–1334
Sabieri M, Utiger RD (1975) Augmentation of thyrotropin responses to thyrotropin releasing hormone following small decreases in serum thyroid hormone concentrations. J Clin Endocrinol Metab 40: 435–440
Chopra II (1981) New insight into metabolism of thyroid hormones. In: Brewer GJ, Eisvogel VP, Grover R Hirschhorn K, Kety SS, Underfriend S, Uhr JW (eds) Physiopathology of endocrine diseases and mechanisms of hormone action, A. R. Liss, New York, pp 67–80
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Kvetny, J. Nuclear thyroxine and triiodothyronine receptors in human mononuclear cells in diabetes mellitus. Diabetologia 24, 428–432 (1983). https://doi.org/10.1007/BF00257341
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DOI: https://doi.org/10.1007/BF00257341