Skip to main content

In vitro culture of epithelial cells derived from urogenital tissues


A tissue culture procedure is described which yields a high percentage of successful longterm cultures of epithelial cells derived from malignant and non-malignant human urogenital tissues.

This is a preview of subscription content, access via your institution.


  1. 1.

    Bregman, R. U., Bregman, E. T.: Tissue culture of benign and malignant human genitourinary tumors. J. Urol. 86, 642–649 (1961)

    Google Scholar 

  2. 2.

    Bubenik, J., Barešová M., Viklický, V., Jakoubková, J., Sainerová, H., Donner, J.: Established cell line of urinary bladder carcinoma (T24) containing tumor-specific antigen. Int. J. Cancer 11, 765–773 (1973)

    Google Scholar 

  3. 3.

    Fraley, E. E., Ecker, S., Vincent, M. M.: Spontaneous in vitro neoplastic transformation of adult human prostatic epithelium. Science 170, 540–542 (1970)

    Google Scholar 

  4. 4.

    Fraley, E. E., Elliott, A. Y., Castro, A. E., Cleveland, P., Hakala, T., Stein, N.: Ribonucleic acid virus associated with human urothelial tumors: Significance for diagnosis and treatment. J. Urol. 111, 378–381 (1974)

    Google Scholar 

  5. 5.

    Hicks, R. M.: The fine structure of the transitional epithelium of rat ureter. J. Cell. Biol. 26, 25–48 (1965)

    Google Scholar 

  6. 6.

    Hosick, H. L.: A note on growth patterns of epithelial tumor cells in primary culture. Cancer Res. 34, 259–261 (1974)

    Google Scholar 

  7. 7.

    Koss, L. G.: The asymmetric unit membranes of the epithelium of the urinary bladder of the rat. An electron microscopic study of the Mechanism of maturation and function. Lab. Invest. 21, 154–168 (1969)

    Google Scholar 

  8. 8.

    Paulson, D.F., Bonar, R.A., Mickey, D.D., Vergara, J., Stone, K.R., Glenn, J.F.: Biology of transitional malignancy. Trans. of the G-U Surg. (in press)

  9. 9.

    Peterson, L. J., Paulson, D. F., Bonar, R. A.: Response of human urothelium to chemical carcinogens in vitro. J. Urol. 111, 154–159 (1974)

    Google Scholar 

  10. 10.

    Porter, K. R., Kenyon, K., Badenhausen, S.: Specializations of the unit membrane. Protoplasma 63, 262–274 (1967)

    Google Scholar 

  11. 11.

    Rigby, C. C., Franks, L. M.: A human tissue culture cell line from a transitional cell tumor of the urinary bladder: Growth, chromosome pattern and ultrastructure. Brit. J. Cancer 24, 746–754 (1970)

    Google Scholar 

  12. 12.

    Stonington, O. G., Hemmingsen, H.: Culture of Cells as a monolayer derived from the epithelium of the human prostate: A new cell growth technique. J. Urol. 106, 393–400 (1971)

    Google Scholar 

  13. 13.

    Yajima, T.: Monolayer culture of human urinary bladder tumors. Jap. J. Urol. 59, 128–134 (1968)

    Google Scholar 

Download references

Author information



Additional information

Supported by N.I.H. Grant No. 1 R26 CA15972-01 and Veterans Administration Projects 1044 and 7080.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Stone, K.R., Paulson, D.F., Bonar, R.A. et al. In vitro culture of epithelial cells derived from urogenital tissues. Urol. Res. 2, 149–153 (1975).

Download citation

Key words

  • Tissue culture
  • prostate
  • bladder
  • kidney