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Structure-potential dependence of adsorbed enzymes and amino acids revealed by the surface enhanced Raman effect

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Abstract

Surface enhanced Raman scattering (SERS) of some enzymes (alkaline phosphatase, horseradish peroxidase and lactoperoxidase) and some amino acids (tryptophan, tyrosine and phenylalanine) on silver electrodes has been studied. The spectral band intensities of certain amino acids and amino acid residues were determined by their orientation on the surface and depended on the electrode potential (E).

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Abbreviations

SERS:

surface enhanced Raman scattering

Trp:

tryptophan

Tyr:

tyrosine

Phe:

phenylalanine

E:

electrode potential

ORC:

oxidation-reduction cycle

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Authors and Affiliations

  1. Institute of Biochemistry, Lithuanian Academy of Sciences, Vilnius, SU-232021 USSR, Russia

    G. -J. A. Vidugiris, A. V. Gudavičius, V. J. Razumas & J. J. Kulys

Authors
  1. G. -J. A. Vidugiris
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  2. A. V. Gudavičius
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  3. V. J. Razumas
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  4. J. J. Kulys
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Vidugiris, G.J.A., Gudavičius, A.V., Razumas, V.J. et al. Structure-potential dependence of adsorbed enzymes and amino acids revealed by the surface enhanced Raman effect. Eur Biophys J 17, 19–23 (1989). https://doi.org/10.1007/BF00257142

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  • DOI: https://doi.org/10.1007/BF00257142

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