Cancer Chemotherapy and Pharmacology

, Volume 13, Issue 2, pp 145–147 | Cite as

Expression of cell surface P-glycoprotein by an Adriamycin-resistant murine fibrosarcoma

  • Raffaella Giavazzi
  • Norbert Kartner
  • Ian R. Hart
Short Communication P-Glycoprotein, Adriamycin Resistance, Murine Fibrosarcoma


Analysis of the cell membrane of Adriamycin (doxorubicin)-resistant UV-2237 ADMR murine fibrosarcoma cells revealed a 170,000-dalton component that is not found in the drug-sensitive parent or revertant cells. Immunoblot (Western blot) analysis showed that this component is similar to the 170,000-dalton P-glycoprotein found on the surface of Chinese hamster ovary cells that exhibit multidrug resistance. Thus, multidrug resistance and P-glycoprotein expression apparently can occur in a wide variety of cells, including the metastatic murine solid tumor cell line described here.


Western Blot Doxorubicin Solid Tumor Tumor Cell Line Multidrug Resistance 
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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Raffaella Giavazzi
    • 1
  • Norbert Kartner
    • 2
  • Ian R. Hart
    • 1
  1. 1.Cancer Metastasis and Treatment Laboratory, LBI-Basic Research ProgramNCI-Frederick Cancer Research FacilityFrederickUSA
  2. 2.Ontario Cancer Institute, Princess Margaret Hospital, and Department of Medical BiophysicsUniversity of TorontoTorontoCanada

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