Abstract
The primary and secondary structure of herpes simplex virus type 1 (HSV-1), varicella-zoster (VZV) and Epstein-Barr virus (EBV) DNA polymerases was calculated by means of computer programs. The comparison of HSV-1 polymerase (pol) sequence to the known primary and tertiary structure of E. coli DNA pol I revealed five short homologous sequences, one of which coincided with the α-helical structure of the DNA-binding domain of E. coli DNA pol. Comparison by primary and secondary structure computer programs of the three DNA polymerases coded by herpesviruses HSV-1, VZV and EBV led to the identification of polypeptide sequences shared by the three DNA pols. In a similar way, the secondary structure of the DNA pol polypeptide in the vicinity of the mutation leading to PAA resistance in HSV-1 DNA pol helped to identify the role of this sequence in the binding of phosphate donated by the nucleoside triphosphate molecule which binds to the DNA pol. Although the computer secondary structure programs are about 60% accurate, it was possible to obtain new information on the properties of certain domains in the DNA polymerases of HSV-1, VZV and EBV.
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Abbreviations
- aa:
-
amino acids
- EBV:
-
Epstein-Barr virus
- HSV-1:
-
herpes simplex virus type 1
- Mr :
-
molecular weight
- PAA:
-
phosphonoacetic acid
- PFA:
-
phosphonoformic acid
- pol:
-
polymerase
- VZV:
-
varicella-zoster virus
References
Gibbs J.S., Chiou H.C., Hall J.D., Mount D.W., Retondo M.J., Weller S.K. & Coen D.M., Proc Nat Acad Sci USA 82, 7969–7973, 1985.
Quinn J.P. & McGeoch D.J., Nucl Acids Res 13, 8143–8163, 1985.
Knopf C., Nucl Acids Res 14, 8225–8226, 1986.
Knopf. J. Gen Virol. 68, 1429–1433, 1987.
Earl P.L., Jones E.V. & Moss B., Proc Nat Acad Sci USA 83, 3659–3663, 1986.
Coen D.M., Furman P.A., Aschman D.P. & Schaffer P.A., Nucl Acid Res 11, 5287–5297, 1983.
Frank K.B., Derse D.D., Bastow K.F. & Cheng Y.C., J Biol Chem 259, 13282–13286, 1984.
Krokau H., Schaffer P. & DePamphilis M.L., Biochemistry 18, 4431–4433, 1979.
Davison A.J. & Scott J.E., J Gen Virol 67, 1759–1816, 1986.
Argos P., Tucker A.D. & Philipson L., Virology 149, 208–216, 1986.
Kyte J. & Doolittle R.F., J Mol Biol., 157, 105–132, 1982.
Hopp T.P. & Woods K.R., Proc Nat Acad Sci USA 78, 3824–3828, 1981.
Ollis D.L., Brick P., Hamlin R., Xuong N.G. & Steitz T.A., Nature 313, 762–766, 1985.
Chou P.Y. & Fasman G.D., Adv Enzymol 47, 45–147, 1978.
Garnier J., Osguthorpe D.J. & Robson B., J Mol Biol 120, 97–120, 1978.
Ollis D.L., Kline C. & Steitz T.A., Nature 313, 818–819, 1985.
Wolf H., Modrow S., Motz M., Jameson B., Hermann G. & Fortsch B., Cabios (in press).
Baer R., Bankier A.T., Biggin M.D., Deininger P.L., Farrell P.J., Gibson T.G., Hatfull G., Hudson G.S., Satchwell S.C., Seguin C., Tuffnell P.S. & Barrell B.G., Nature 310, 207–211, 1984.
Needleman S.B. & Wunsch C.D., J Mol Biol 48, 443–453, 1970.
Joyce C.M., Kelley W.S. & Grindley N.D.F., J Biol Chem 257, 1958–1964, 1982.
Brown W.E., Stump K.H. & Kelley W.S., J Biol Chem 257, 1965–1972, 1982.
Devereux J., Haeberlin P. & Smithies O., Nucl Acid Res 12, 387–395, 1984.
Maizel J.V. & Lenk R.P., Proc Nat Acad Sci 78, 7665–7669, 1981.
McKeon F.D., Kirschner M.W. & Caput D., Nature 319, 463–468, 1986.
Shlomai J., Asher Y., Gordon Y.J., Olshevsky U. & Becker Y., Virology 66, 330–335, 1975.
Pabo C.O. & Sauer T., Ann Rev Biochem 53, 293–321, 1984.
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Requests for reprints should be addressed to Professor Yechiel Becker, Department of Molecular Virology, Faculty of Medicine, The Hebrew University, P.O. Box 1172, 91010 Jerusalem, Israel.
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Becker, Y. Computer-assisted primary and secondary structure analyses of DNA polymerases of herpes simplex, Epstein-Barr and varicella zoster viruses reveal conserved domains with some homology to DNA-binding domain in E. coli DNA pol I. Virus Genes 1, 351–367 (1988). https://doi.org/10.1007/BF00257098
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DOI: https://doi.org/10.1007/BF00257098