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Extractive bioconversion in aqueous two-phase systems

Production of prednisolone from hydrocortisone using Arthrobacter simplex as catalyst

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The transformation of hydrocortisone to prednisolone was studied in aqueous two-phase systems, as a model for the extractive bioconversion of fine chemicals. The bacterium, Arthrobacter simplex, was able to grow in the two-phase system and the cells could be revitalized after a period of use. Use of aqueous two-phase systems made it possible to operate the reaction at higher substrate concentrations than in pure buffer solution. An adsorptive method to remove the product from the top phase was tested and shown to be both efficient and compatible with the overall process. In order to reduce the costs of operation in aqueous two-phase systems, a cheaper starch-based polymer, Reppal-PES, was successfully used as a substitute for dextran.

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Dedicated to Professor Dr. Georg Manecke on the occasion of his 70th birthday

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Kaul, R., Mattiasson, B. Extractive bioconversion in aqueous two-phase systems. Appl Microbiol Biotechnol 24, 259–265 (1986).

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