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Canine urinary bladder epithelial cells: Preparation for cell culture by enzyme dispersion

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Summary

In a qualitative and quantitative study of enzymic dispersion of cells from the mucosal layer stripped from canine urinary bladder, trypsin was found to be equal or superior to the other enzymes tested for dispersal of urothelial cells specifically. Collagenase or collagenase plus trypsin served to disperse the whole tissue. A procedure for recovering the urothelial cells as a single-cell suspension and establishing them in culture is presented. The morphology, culture behaviour, and chromosome complement of these cells is described.

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Authors and Affiliations

  1. Veterans Administration Hospital, Durham, North Carolina, USA

    Robert A. Bonar Ph. D., Charles F. Reich III & Yousuf Sharief

  2. Division of Urology, Department of Surgery, Duke University Medical Center, Durham, North Carolina, USA

    Robert A. Bonar Ph. D., Charles F. Reich III & Yousuf Sharief

Authors
  1. Robert A. Bonar Ph. D.
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  2. Charles F. Reich III
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  3. Yousuf Sharief
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Bonar, R.A., Reich, C.F. & Sharief, Y. Canine urinary bladder epithelial cells: Preparation for cell culture by enzyme dispersion. Urol. Res. 5, 87–94 (1977). https://doi.org/10.1007/BF00256846

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  • DOI: https://doi.org/10.1007/BF00256846

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