Summary
The anticholinergic and musculotropic relaxant properties of desmethylimipramine (DMI) on smooth muscle were investigated utilizing cholinergic radioligand receptor binding and in-vitro muscle bath techniques. Receptor binding studies revealed the direct antimuscarinic potency of DMI to be 1/480th that of atropine. Muscle bath studies characterized discrete antimuscarinic and musculotropic actions. An initial, competitive antimuscarinic action could be separated from a delayed onset, noncompetitive musculotropic action by altering the time between the addition of DMI and the addition of bethanechol to the smooth muscle bath.
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This work was supported in part by grants from the Veterans Administration, by NIH Grant #RO-2-AM-2-6508-04, and by the McCabe Fund
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Levin, R.M., Staskin, D.R. & Wein, A.J. Analysis of the anticholinergic and musculotropic effects of desmethylimipramine on the rabbit urinary bladder. Urol. Res. 11, 259–262 (1983). https://doi.org/10.1007/BF00256342
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DOI: https://doi.org/10.1007/BF00256342