Summary
While chemotherapy offers a valuable adjunct to surgery in the management of intravesical bladder cancer, an accurate in vitro predictive test for chemosensitivity has yet to be developed. Drug sensitivity of the human bladder cancer cell line J-82 was assessed using monolayer, stem cell and [3H]thymidine incorporation assays. The 72-h monolayer assay provided a rapid reflection of in vitro drug sensitivity and when combined with the labeling index the results generally paralleled those obtained with the soft agar stem cell assay without the associated large commitment of time and labor. It is suggested that 72-h monolayer assay alone or in combination with [3H]thymidine labeling index may offer valuable insight into the chemotherapeutic response of bladder tumors.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Asahi T, Matsumura Y, Tanakashi T, Yoshimoto J, Kaneshige T, Fugita Y, Okmori H (1980) The effects of intravesical instillation of thiotepa on the recurrent rate of bladder tumors. ACTA Med Okay 34:43–39
Ballou RJ, Tseng MT (1983) Improved method for processing autoradiographs of monolayer cells grown on multiwell plates. In Vitro 19:805–806
Basera R (1965) The relationship of the cell cycle to tumor growth and control of cell division. Cancer Res 25:581–595
Burnand KG, Boyd PJR, Mayo ME, Shuttleworth KED, Lloyd-Davies RW (1976) Single dose intravesical thioterpa as an adjunct to cystodiathermy in the treatment of transitional cell bladder cancer. Br J Urol 48:55–58
Hamburger AW (1981) Use of in vitro tests in predictive cancer chemotherapy. J Natl Cancer Inst 66:981–988
Hamburger AW, Salmon SE (1977) Primary bioassay of human tumor stem cells. Science 197:461–463
Holmes HL, Little JM (1974) Tissue culture microtext for predicting response of human cancers to chemotherapy. Lancet II:985–987
Kato T, Nishimoto T, Kumagi I, Miura K (1979) Chemosensitivity of human bladder cancer cells in long-term culture and clinical responses to selected anticancer drugs. Cancer 44:658–663
Koontz WW (1979) Intravesical chemotherapy and chemoprevention of superficial low grade, low stage bladder carcinoma. Semin Oncol 6:217–219
Nieh PT, Daly JA, Heaney NM, Prout GR (1978) The effect of thio-TEPA on normal and tumor urothelium. J Urol 119:59–61
Pavone-Macaluso M, Gebia N, Biondo F, Rizzo FP, Caramio G, Rausa L (1973) Experimental techniques for testing the sensitivity of bladder tumors to antineoplastic drugs. Urol Res 1: 60–66
Roper PR, Drewinko B (1976) Comparison of in vitro methods to determine drug-induced cell lethality. Cancer Res 36:2182–2188
Rupniak HT, Hall BT (1980) The poor cloning ability in agar of human tumour cells from biopsies of primary tumours. Cell Biol Int Rep 4:479–486
Safa AR, Tseng MT (1982) Preparing autoradiographs of monolayer cell cultures grown in polystyrene multiwell plates for scoring labeling indices. Stain Technol 57:319–321
Safa AR, Tseng MT, Ballou RJ (1983) Sequential study of the influence of adriamycin on cell proliferation and ultrastructure of cultured mammary tumor cells. Proc Soc Exp Biol Med 174:276–283
Salmon SE, Hamburger AW, Soehnlen B, Durie BG, Alberts DS, Moon TE (1978) Quantitation of differential sensitivity of human tumor stem cells to anticancer drugs. N Engl J Med 298:1321–1327
Shrivastav S, Paulson DF (1980) In vitro chemotherapy testing of transitional cell carcinoma. Invest Urol 17:395–400
Simpson WG, Ballou RJ, Tseng MT (1985) Determination of thymidine labeling indices of cultured cells grown on detached chamber slides. Cell Tissue Kinet 18:155–157
Sky-Peck HH (1971) Effects of chemotherapy on the incorporation of 3H-thymidine into DNA of human neoplastic tissue. Natl Cancer Inst Monograph 34:197–204
Slack NH, Prout GR (1980) The heterogeneity of invasive bladder carcinoma and different responses to treatment. J Urol 123:644–652
Socquet Y (1981) Surgery and adjuvant chemotherapy with high dose methotrexate and folinic acid for infiltrating tumor of the bladder. Cancer Treat Rep 65:187–189
Sondak VK, Bertelsen CA, Tanigawa N, Hildebrandj-Zanki SU, Morton DL, Korn EL, Kern DH (1984) Clinical correlations with chemosensitivites measured in a rapid thymidine incorporation assay. Cancer Res 44:1725–1728
Tseng MT, Safa AR (1983) In vitro drug screening and individualized treatment planning for cancer patients. Cancer Detect Prev 6:371–379
Weaver D, Khare N, Haigh J, Adelstein E, Osenandler R, Ross G (1980) The effect of chemotherapeutic agents on the ultrastructure of transitional cell carcinoma in tissue culture. Invest Urol 17:288–292
Weisenthal LM, Lalude AO, Miller JB (1983) In vitro chemosensitivity of human bladder cancer. Cancer 51:1490–1496
Wright JC, Cob JP, Gumport SL, Golomb FM, Safadi D (1957) Investigation of the relation between clinical and tissue culture response to chemotherapeutic agents on human cancer. N Engl J Med 247:1207–1211
Yoshimoto J, Matsumura Y, Tanahasi T, Ohmori H, Tokiwa T, Sato J (1982) Effects of steroid sex hormones and adriamycin on human bladder cancer cell lines in culture. ACTA Med Okay 36:61–66
Zilkak S, Osland ME, McCaffrey R (1978) Effect of inhibitions of plant cell division on mammalian tumor cells in vitro. Cancer Res 41:1879–1883
Author information
Authors and Affiliations
Additional information
Supported in part by a grant from the Commission on Academic Excellence and a Cancer Research Fellowship from the University of Louisville
Rights and permissions
About this article
Cite this article
Anderson, K.C., Simpson, W.G., Ballou, R.J. et al. In vitro chemosensitivity of J-82 human bladder cancer cells. Urol. Res. 14, 141–144 (1986). https://doi.org/10.1007/BF00255833
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00255833