Abstract
We have investigated the effect of bulk viscosity on lipid translational diffusion using the excimer formation technique. In contrast to a study by Vaz et al. (1987), performed with the fluorescence recovery after photobleaching technique, we observed only a minor decrease of less than a factor of two for pyrene labelled phosphatidylcholine in glycerinated phosphatidylcholine bilayer membranes compared to an aqueous dispersion. Even the diffusion of pyrene labelled gangliosides with an oligosaccharide head-group that protrudes from the membrane surface is not strongly restricted by the increased bulk viscosity. We conclude that the viscosity of the fluid bounding the lipid bilayers is of minor importance for the diffusion of membrane lipids.
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Abbreviations
- DPPC:
-
1-2 dipalmitoyl-sn-glycero-3-phosphocholine
- DSPC:
-
1-2 distearoyl-sn-glycero-3-phosphocholine
- PyPC:
-
1-acyl-2-[10(-1-pyrene)decanoyl]-sn-glycero-3-phosphocholine
- PyGM1 :
-
N-12-(1-pyrene)dodecanoyl-lyso GM1
- PyGM2 :
-
N-12-(1-pyrene)dodecanoyl-lyso GM2
- PyGM3 :
-
N-12-(1-pyrene) dodecanoyl-lyso GM3
- IM :
-
fluorescence intensity of the monomeric pyrene probe
- ID :
-
fluorescence intensity of the excimer
References
Clegg RM, Vaz WLC (1985) Translational diffusion of proteins and lipids in artificial lipid bilayer membranes. A comparison of experiment with theory. In: Watts A, De Pont JJHHM (eds) Progress in protein-lipid interactions. Elsevier Science, Amsterdam, pp 173–229
Cohen MH, Turnbull D (1959) Molecular transport in liquids and glasses. J Chem Phys 31:1164–1169
Galla HJ, Hartmann W (1980) Excimer-forming lipids in membrane research. Chem Phys Lipids 27:199–219
Galla HJ, Hartmann W (1981) Pyrene decanoic acid and pyrene lecithin. Methods Enzymol 72:471–479
Galla HJ, Sackmann E (1974) Lateral diffusion in the hydrophobic region of membranes: Use of pyrene excimers as optical probes. Biochim Biophys Acta 339:103–115
Galla HJ, Hartmann W, Theilen U, Sackmann E (1979) On twodimensional passive random walk in lipid-bilayers and fluid channels of biomembranes. J Membr Biol 48:215–236
Hresko RC, Sugar JP, Barenholz Y, Thompson TE (1986) Lateral distribution of a pyrene labeled phosphatidylcholine in phosphatidylcholine bilayers. Fluorescence phase and modulation study. Biochemistry 25:3813–3828
Hughes BD, Pailthorpe BA, White LR (1981) The translational and rotational drag on a cylinder moving in a membrane. J Fluid Mech 110:349–372
Hughes BD, Pailthorpe BA, White LR, Sawyer WH (1982) Extraction of membrane microviscosity from translational and rotational diffusion coefficients. Biophys J 37:673–676
Landolt-Börnstein (1969) Zahlenwerte und Funktionen. 6. Aufl. Band II: Eigenschaften der Materie in ihren Aggregatzuständen. Teil 5a: Transportphänomene I. Springer, Berlin Heidelberg New York
McDaniel RV, McIntosh TJ, Simon SA (1983) Nonelectrolyte substitution for water in phosphatidylcholine bilayers. Biochim Biophys Acta 731:97–108
Müller HJ, Galla HJ (1987) Chain length and pressure dependence of lipid translational diffusion. Eur Biophys J 14:485–492
Ollmann M, Schwarzmann G, Sandhoff K, Galla HJ (1987) Pyrene labeled gangliosides. Micelle formation in aqueous solution, lateral diffusion and thermotropic behaviour in phosphatidylcholine bilayers. Biochemistry 26:5943–5952
Peters R, Beck K (1983) Translational diffusion in phospholipid monolayers measured by fluorescence microphotolysis. Proc Natl Acad Sci USA 80:7183–7187
Saffman PG (1976) Brownian motion in thin sheets of viscous fluid. J Fluid Mech 73:593–602
Saffman PG, Delbrück M (1975) Brownian motion in biological membranes. Proc Natl Acad Sci USA 72:3111–3113
Schwarzmann G, Sandhoff K (1987) Lysogangliosides: Synthesis and use in preparing labeled gangliosides Methods. Methods Enzymol 138:319–341
Vaz WLC, Hallmann D (1983) Experimental evidence against the applicability of the Saffman-Delbrück model to the translational diffusion of lipids in phosphatidylcholine bilayer membranes. FEBS Lett 152:287–290
Vaz WLC, Criado M, Madeira VMC, Schoellmann G, Jovin TM (1982) Size dependence of the translational diffusion of large integral membrane proteins in liquid-crystalline phase lipid bilayers. A study using fluorescence recovery after photobleaching. Biochemistry 21:5608–5612
Vaz WLC, Clegg RM, Hallmann D (1985) Translational diffusion of lipids in liquid crystalline phase phosphatidylcholine multibilayers. A comparison of experiment with theory. Biochemistry 24:781–786
Vaz WLC, Stumpel J, Hallmann D, Gambacorta A, De Rosa M (1987) Bounding fluid viscosity and translational diffusion in a fluid bilayer. Eur Biophys J 15:111–115
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Ollmann, M., Robitzki, A., Schwarzmann, G. et al. Minor effects of bulk viscosity on lipid translational diffusion measured by the excimer formation technique. Eur Biophys J 16, 109–112 (1988). https://doi.org/10.1007/BF00255520
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DOI: https://doi.org/10.1007/BF00255520