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Thyrotropin releasing hormone (TRH)-immunoreactive cell groups in the rat central nervous system

Experimental Brain Research volume 68, pages 213–217 (1987)Cite this article

Summary

Using a paraformaldehyde-picric acidglutaraldehyde-containing fixative and treatment of the tissue with sodium borohydride, numerous and widespread TRH-immunoreactive cell bodies were observed in the central nervous system of colchicinetreated rats, including the olfactory bulb, cortical and hippocampal areas, the caudate nucleus and other subcortical areas, many hypothalamic nuclei, the periaqueductal central gray, pontine nuclei, medulla oblongata and the dorsal horn of the spinal cord. Most of these cells could not be visualized with the same antiserum when conventional fixation methods based on formalin alone were used. The present findings suggest that TRH systems are considerably more extensive than hitherto assumed.

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Affiliations

  1. Department of Histology, Karolinska Institute, P.O. Box 60400, S-10401, Stockholm, Sweden

    Y. Tsuruo, T. Hökfelt & T. Visser

  2. Department of Medicine, Erasmus University, Rotterdam, The Netherlands

    Y. Tsuruo, T. Hökfelt & T. Visser

Authors
  1. Y. Tsuruo
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  2. T. Hökfelt
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  3. T. Visser
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Tsuruo, Y., Hökfelt, T. & Visser, T. Thyrotropin releasing hormone (TRH)-immunoreactive cell groups in the rat central nervous system. Exp Brain Res 68, 213–217 (1987). https://doi.org/10.1007/BF00255248

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  • DOI: https://doi.org/10.1007/BF00255248

Key words

  • Neuropeptide
  • Brain
  • Spinal cord
  • Immunohistochemistry