Summary
The purpose of this study was to demonstrate the ability of a new method to discriminate between benign and malignant tissue. Tissue samples from three patients with bladder tumours and one patient with bladder diverticula have been examined by histophotometry. This method, based on the correlation of nuclear volume and nucleic acid contents per cell uses thick formalin fixed samples. The results are obtained by measurements averaging more than one hundred thousand cells per sample. By this histophotometric examination a clear differentiation between malignant and benign tissue could be achieved. It was also possible to determine the degree of malignancy and to differentiate between infiltrating and noninfiltrating tumour, as well as inflamed bladder tissue. The results obtained histophotometrically were compared with the histological findings.
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References
Casperson, T.: Über den chemischen Aufbau der Strukturen des Zellkernes. Scandinavian Archives of Physiology 73, Syppl. 8 (1963)
Chandrasekhar, S.: Radiative Transfer. New York: Dover Publications 1960
Goerttler, K., Haag, D., Tasca, C.: Cytophotometrische Untersuchungen an Zellkernen von experimentell erzeugten Neoplasmen. Zeitschrift für Krebsforschung 76, 155 (1971)
Koller, P. C.: The nucleus of the cancer cell. Experimental Cell Research (Suppl.) 9, 3 (1963)
Koss, G. L., Bartels, P. H., Bibbo, M., Freed, S. Z., Taylor, J., Wied, G. L.: Computer discrimination between benign and malignant urothelial cells. Acta Cytologica 19, 378 (1975)
Leuchtenberger, C., Leuchtenberger, R., Davis, A. M.: A microspectrophotometric study of desoxyribose nucleic acid (DNA) content in cells of normal and malignant human tissues. American Journal of Pathology 30, 65 (1954)
Mullaney, P. F., Dean, P. N.: The small angle light scattering of biological cells. Biophysical Journal 10, 764 (1970)
Pollister, A. W., Ornstein, L.: Analytical cytology. Mellor, C. R. (ed.). New York, and London: McGraw Hill Publications 1959
Sandritter, W., Carl, M., Ritter, W.: Cytophotometric measurements of the DNA content of human malignant tumors by the Feulgen reaction. Acta Cytologica 10, 26 (1966)
Wied, G. L., Bartels, P. H., Bahr, G. F., Oldfield, D. G.: Taxonomic intracellular analytic system (TICAS) for cell identification. Acta Cytologica 12, 180 (1968)
Zetterberg, M. D., Esposti, P. L.: Cytophotometric DNA-analysis of aspirated cells from prostatic carcinoma. Acta Cytologica 20, 46 (1976)
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Kurth, K.H., Binder, A., Jacobi, G.H. et al. Histophotometry: A new method for automated histological examination of solid tissue samples demonstrated on bladder cancer. Urol. Res. 7, 113–118 (1979). https://doi.org/10.1007/BF00254691
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DOI: https://doi.org/10.1007/BF00254691