Skip to main content
SpringerLink
Log in

Therapy of solid Walker carcinosarcoma 256 with bleomycin after synchronization with hydroxyurea

  • Original Articles
  • Synchronization with Hydroxyurea
  • Published:
Cancer Chemotherapy and Pharmacology Aims and scope Submit manuscript

Summary

Rats with the solid Walker carcinosarcoma 256 were synchronized with hydroxyurea (6×50 mg/kg or 1×300 mg/kg body weight) and treated with bleomycin (32 mg/kg body weight) at different time points thereafter. Bleomycin clearly affects Walker carcinosarcoma cells at the G1/S boundary or in S-phase. The improvement in the results of bleomycin therapy after pretreatment with hydroxyurea can mainly be accounted for by the synchronization of the tumour cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Barranco SC, Humphrey RM (1971) The effects of bleomycin on survival and cell progression in Chinese hamster cells in vitro. Cancer Res 31:1218

    Google Scholar 

  2. Barranco SC, Novak JK, Humphrey RM (1973) Response of mammalian cells following treatment with bleomycin and 1,3-bis(2-chloroethyl)-1-nitrosourea during plateau phase. Cancer Res 33:691

    Google Scholar 

  3. Bitter K, Bitter BS (1976) Die Zellzyklus-phasenspezifische Wirkung des Bleomycins an Planttenepithelkarzinomen nach Synchronisation mit Hydroxyharnstoff. Fortschr Kiefer Gesichtschir 21:215

    Google Scholar 

  4. Bruce WR, Mecker BE, Powers WE, Valeriote FA (1969) Comparison of the dose- and time-survival curves for normal hematopoietic and lymphoma colony-forming cells exposed to vinblastine, vincristine, arabinosylcytosine and amethopterin. J Natl Cancer Inst 42:1015

    Google Scholar 

  5. Carter SK (1978) The clinical evaluation of analogues. II. Bleomycins. Cancer Chemother Pharmacol 1:123

    Google Scholar 

  6. Christensen L (1972) Bleomycin, ein neues Antitumor-Antibiotikum zur Behandlung neoplastischer Krankheiten. Wien Klin Wochenschr 84:737

    Google Scholar 

  7. Ganzer U, Nitze HR (1971) Die Synchronisation menschlicher Gewebe in vivo. Der Einfluß der Blockadezeit. Arch Klin Exp Ohr- Nas- Kehlk-Heilk 198:239

    Google Scholar 

  8. Gibson MH, Bertalanffy ED (1972) In vivo synchrony of solid B 16 melanoma by cytosine arabinoside, and inhibitor of DNA synthesis. J Natl Cancer Inst 49:1007

    Google Scholar 

  9. Glupe J, Kraus H, Wannenmacher E (1972) Erfahrungen mit der kombinierten cytostatischen und Strahlenbehandlung unter Nutzung des Synchronisationseffektes bei Geschwülsten im Kopf und Halsbereich. Hals- Nas- Ohrenarzt 20:18

    Google Scholar 

  10. Hartenstein R, Pfundmair K, Possinger K (1977) Partialsyn-chronisation von Tumorzellen in vivo mit Hydroxyharnstoff. Z Krebsforsch 88:261

    Google Scholar 

  11. Hartwich G, Tschätsch W, Lutz H (1973) Zytostatische Kombinationsbehandlung mit Vincristinsulfat und Ifosfamid. Dtsch Med Wochenschr 98:1203

    Google Scholar 

  12. Hermann HJ, Ammon J, Nüvemann M, zum Winkel K (1977) Strahlentherapie und Chemotherapie nach Teilsynchronisation des Zellzyklus. Therapiewoche 27:2052

    Google Scholar 

  13. Hill BT, Baserga R (1975) The cell cycle and its significance for cancer treatment. Cancer Treat Rev 2:159

    Google Scholar 

  14. Ichikawa T, Nakano I, Hirokawa I (1969) Bleomycin treatment of the tumors of penis and scrotum. J Urol 102:699

    Google Scholar 

  15. Iversen OH, Clausen OP, Iversen UM, Rohrbach R (1976) Some effects of bleomycin on the proliferation, maturation time and protein synthesis of hairless mouse epidermis. Cell Tissue Kinet 9:77

    Google Scholar 

  16. Jedrzejezak WW, Siekierzynski M, Dziuk E, Czarnecki G, Bielec M (1976) Combination of amethopterine (MTX) and cyclophosphamide (CPM) based on the principle of partial synchronization of cell proliferation in lung cancer. Phase II study. Arch Geschwulstforsch 46:471

    Google Scholar 

  17. Jellinghaus W, Maidhof R, Schultze B, Maurer W (1975) Experimentelle Untersuchungen und zellkinetische Berechnungen zur Frage der Synchronisation mit Vincristin in vivo (Mäuseleukämie L 1210, Krypten-Epithelien der Maus). Z Krebsforsch 84:161

    Google Scholar 

  18. Klein HO, Lennartz KJ, Gross R, Eder M, Fischer R (1972) In vivo- und In vitro-Untersuchungen zur Zellkinetik und Synchronisation menschlicher Tumorzellen. Ihre Bedeutung für die zytostatische Tumortherapie. Dtsch Med Wochenschr 97:1273

    Google Scholar 

  19. Klein HO, Gerecke D, Borberg H, Gross R, Hoefer-Janker H, Scheef W, Diehl V, Lohmann E, Adler D, Buter E, Löhning A, Brock N, Burkert H (1975) Ergebnisse der Synchronisationstherapie mit Vincristin und Cyclophosphamid bei Lymphogranulomatose, Retikulumzell- und Lymphosarkom. Eine Phase II-Studie. Dtsch Med Wochenschr 100:1719

    Google Scholar 

  20. Lampkin BC, Nagao T, Mauer AM (1971) Synchronization and recruitment in acute leukemia. J Clin Invest 50:2204

    Google Scholar 

  21. Madoc-Jones H, Mauro F (1974) Site of action of cytotoxic agents in the cell life cycle. In: Sartorelli AC, Johns DG (eds) Antineoplastic and immunosuppressive agents, Part I. Springer-Verlag, Berlin Heidelberg New York, p 205

    Google Scholar 

  22. Mattern J, Wayss K, Volm M (1978) Improvement of therapeutic effects of cytosine arabinoside on Walker-carcinosarcoma after different synchronization with hydroxyurea. Eur J Cancer 14:603

    Google Scholar 

  23. Müller WEG, Totsuka A, Zahn RK, Unezawa H (1975) Methode zur Bestimmung der Bleomycin-inaktivierenden Enzymaktivität in Geweben. Z Krebsforsch 83:151

    Google Scholar 

  24. Nicolini C (1976) The principles and methods of cell synchronization in cancer chemotherapy. Biochem Biophys Acta 458:243

    Google Scholar 

  25. Pouillart P, Weiner R, Schwarzenberg L, Misset JL, Oldham R, Amiel JL, Mathé G (1975) Combination chemotherapy based on a model of cell recruitment by partial synchronization. Med Pediatr Oncol 1:123

    Google Scholar 

  26. Putten LM van, Keizer HJ, Mulder JH (1976) Perspectives in cancer research. Synchronization in tumour chemotherapy. Eur J Cancer 12:79

    Google Scholar 

  27. Rajewsky MF, Hülser DF, Fabricius E (1971) Untersuchungen zur Synchronisation in vivo: Temporäre Inhibition der DNA-Synthese durch Hydroxyharnstoff in normalen und malignen Säugerzellsystemen. Z Krebsforsch 76:266

    Google Scholar 

  28. Sauer H, Wilmanns W (1976) Derzeitiger Stand der Synchronisationstherapie von malignen Tumoren und akuten Leukämien. Klin Wochenschr 54:197

    Google Scholar 

  29. Schuhmacher J, Kampmann H, Mattern J, Volm M, Wayss K, Zimmerer J (1974) Incorporation of 131Iododeoxyuridine into the DNA of tumour-bearing rats after partial synchronization as a tool in scintigraphic tumor localization. Nuklearmedizin 12:309

    Google Scholar 

  30. Sinclair WK (1967) Hydroxyurea: Effects on Chinese hamster cells grown in culture. Cancer Res 27:297

    Google Scholar 

  31. Suzuki H, Nagai K, Jamaki H, Tanaka N, Umezawa H (1968) Mechanism of action of bleomycin. Studies with the growing culture of bacterial and tumor cells. J Antibiot 21A:379

    Google Scholar 

  32. Terasima T, Yasukawa M, Umezawa H (1970) Treaks and rejoining of DNA in cultured mammalian cells treated with bleomycin. Gann 61:512

    Google Scholar 

  33. Tobey RA (1972) Arrest of Chinese hamster cells in G2 following treatment with the anti-tumor drug bleomycin. J Cell Physiol 79:259

    Google Scholar 

  34. Umezawa H, Takeuchi T, Hori S, Sawa T, Ishizuka M, Ichikawa T, Komai T (1972) Studies on the mechanism of antitumor effect of bleomycin on squamous cell carcinoma. J Antibiot 25A:409

    Google Scholar 

  35. Volm M (1977) Die Synchronisation als ergänzendes Therapieprinzip bei der Behandlung maligner Tumoren. Theoretische und tierexperimentelle Grundlagen. Therapiewoche 27:8009

    Google Scholar 

  36. Volm M, Wayss K (1975) Keine Verbesserung der Chemotherapie beim soliden Neurosarkom durch Teilsynchronisation mit Hydroxyharnstoff. Dtsch Med Wochenschr 100:2102

    Google Scholar 

  37. Volm M, Krieg L, Mattern J, Wayss K (1977) Effect of synchronization on chemotherapy of solid transplanted tumours. Eur J Cancer 13:1099

    Google Scholar 

  38. Volm M, Krieg L, Mattern J, Wayss K (1979) Chemotherapie bei soliden Transplantationstumoren nach Synchronisation. Strahlentherapie 155:137

    Google Scholar 

  39. Wannenmacher M, Esser E, Glupe J, Schumann J (1974) Klinische und experimentelle Untersuchungen zur Strahlenbehandlung inoperabler Tumoren nach Teilsynchronisation. Strahlentherapie 147:1

    Google Scholar 

  40. Watanabe M, Takabe Y, Katsumata T, Terasima T (1974) Effects of bleomycin on progression through the cell cycle of mouse l-cells. Cancer Res 34:878

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Experimental Pathology, German Cancer Research Center, Im Neuenheimer Feld 280, D-6900, Heidelberg 1, Federal Republic of Germany

    M. Volm, J. Mattern, N. Weber & K. Wayss

Authors
  1. M. Volm
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Mattern
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. N. Weber
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. Wayss
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Volm, M., Mattern, J., Weber, N. et al. Therapy of solid Walker carcinosarcoma 256 with bleomycin after synchronization with hydroxyurea. Cancer Chemother. Pharmacol. 4, 89–93 (1980). https://doi.org/10.1007/BF00254028

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00254028

Keywords

Search

Navigation