Summary
Incubation in vitro of human red cells in increasing glucose concentrations results in a rise in both haemoglobin A1c levels and an intermediate band when measured by an isoelectric focussing method. There are strong correlations between blood glucose levels, levels of haemoglobin A1c and the intermediate band both in vitro and in blood samples taken from diabetic patients. As the intermediate band is also included in the measurement of haemoglobin A1c by the usual analytical methods, this may lead to inaccurate results.
Article PDF
Similar content being viewed by others
References
Mortensen HB (1980) Steady levels of haemoglobin A1c concentration during short time fluctuations in blood glucose. Sci Tools 27:21–22
Svendsen PA, Christiansen JS, Soegaard U, Welinder BS, Nerup J (1980) Rapid changes in chromatographically determined haemoglobin A1c. Induced by short term changes in glucose concentration. Diabetologia 19:130–136
Svendsen PA (1979) Fast glycosylation of haemoglobin. Lancet 1:603
Goldstein DE, Peth SB, England JD (1980) Effects of acute changes in blood glucose on HbA1c. Diabetes 29:623–628
Abraham EC, Huff TA, Cope ND (1978) Determination of glycosylated haemoglobins with a new microcolumn procedure. Diabetes 27:931–937
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Stickland, M.H., Perkins, C.M. & Wales, J.K. The measurement of haemoglobin A1c by isoelectric focussing in diabetic patients. Diabetologia 22, 315–317 (1982). https://doi.org/10.1007/BF00253573
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00253573