Abstract
Rat kidney tissue homogenates, incubated in vitro with 99Tc-DMS were subjected to differential centrifugation. Specific radioactivities of subcellular fractions were as follows: cytosol 13,552, microsomes 3,600, mitochondria 2,707 cpm/mg protein, and nuclei 2,642 cpm/mg DNA.
The cytosol fraction was further analyzed by ion-exchange chromatography on DEAE-Sephadex A-25 column. In this way it was separated into several distinct protein zones, three of them bearing a significant amount of 99Tc-DMS preparation. The highest specific radioactivities of proteins were eluted from the DEAE-Sephadex column with 30 mmol/l and 50–110 mmol/l NaCl solution.
Total nucleic acids isolated from purified nuclei, mitochondria and microsomes contained a certain amount of bound 99Tc-DMS complex. Nuclear nucleic acids had the highest specific radioactivity, suggesting that 99Tc-DMS complex penetrates into the nuclei.
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Petrović, J., Vanlić-Razumenić, N. Biochemical studies of the renal radiopharmaceutical compound dimercaptosuccinate. Eur J Nucl Med 7, 308–310 (1982). https://doi.org/10.1007/BF00253426
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DOI: https://doi.org/10.1007/BF00253426