Summary
Protoplasts of Brevibacterium flavum cultured in a medium containing 50 μg·l-1 of biotin were prepared with lysozyme and immobilized in matrices of agar-acetylcellulose filters. The immobilized protoplasts were applied to l-glutamate production from glucose and urea in a batch system. The productivity of l-glutamate by the immobilized protoplasts was 2.5 times higher than that by immobilized whole cells under optimal conditions. Maximal productivity initially reached 1.5 mg·ml-1. The immobilized protoplasts of B. flavum could be used six times for l-glutamate production with retention of about 70% of the initial productivity.
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References
Bernt E, Bergmeyer HU (1974) l-Glutamate UV-assay with glutamate dehydrogenase and NAD. In: Bergmeyer HU (ed) Methods of enzymatic analysis. Academic Press, New York, vol 4, pp 1704–1708
Durand G, Navarro MJ (1978) Immobilized microbial cells. Process Biochem 13:14–23
Hirose Y, Okada H (1979) Microbial production of amino acids. In: Peppler JH, Perlman D (eds) Microbial technology, microbial processes. Academic Press, New York, vol 1, pp 211–240
Karube I, Matsunaga T, Tsuru S, Suzuki S (1976) Continuous hydrogen production by immobilized whole cells of Clostridium butyricum. Biochim Biophys Acta 444:338–343
Kokubu T, Karube I, Suzuki S (1978) α-Amylase production by immobilized whole cells of Bacillus subtilis. Eur J Appl Microbiol Biotechnol 5:233–240
Morikawa Y, Karube I, Suzuki S (1980) Continuous production of bacitracin by immobilized living whole cells of Bacillus sp. Biotechnol Bioeng 222:1015–1023
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Karube, I., Kawarai, M., Matsuoka, H. et al. Production of l-glutamate by immobilized protoplasts. Appl Microbiol Biotechnol 21, 270–272 (1985). https://doi.org/10.1007/BF00252702
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DOI: https://doi.org/10.1007/BF00252702