Summary
Plants were regenerated from callus arising from protoplast fusion of two S. tuberosum diploids. Tetraploid progeny from the fusion of the two diploid partners had increased vigor. Isozyme analysis confirmed the presence of proteins from both partners in the fusion progeny. Pigmentation of tubers and anthers was heightened substantially in the fusion products. This fusion, the first intra-specific fusion within S. tuberosum, indicates that somatic fusion may be useful for transferring traits within this group.
Similar content being viewed by others
References
Austin S, Baer MA, Helgeson JP (1985) Transfer of resistance to potato leaf roll virus from Solatium brevidens into Solanum tuberosum by somatic fusion. Plant Sci Lett 39: 75–82
Baer MA, Austin S, Helgeson JP (1984) Somatic fusions of protoplasts of Solanum species. Plant Physiol (Suppl) 75:133
Evans DA (1983) Agricultural application of plant protoplast fusion. Biotechnology 1:253–261
Galbraith DW, Galbraith JEC (1979) A method for identification of fusion of plants protoplasts derived from tissue cultures. Z Pflanzenphysiol 93: 149–158
Haberlach GT, Cohen BA, Reichert NA, Baer MA, Towill LE, Helgeson JP (1985) Isolation, culture and regeneration of protoplasts from potato and several related Solanum species. Plant Sci Lett 39:67–74
Hager DA, Burgess RA (1980) Elution of proteins from sodium dodecyl/sulphate-polyacrylamide gels, removal of sodium dodecyl sulphate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes. Anal Biochem 109:76–86
Karp A, Nelson RS, Thomas E, Bright SWJ (1982) Chromosome variation in protoplast-derived potato plants. Theor Appl Genet 63: 1–7
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Melchers G, Sacristan MD, Holder AA (1978) Somatic hybrid plants of potato and tomato regenerated from fused protoplasts. Carlsberg Res Commun 43:203–218
Quirios CF (1981) Starch gel electrophoresis technique used with alfalfa and other Medicago species. Can J Plant Sci 61:754–749
Ramulu KS, Dijkhuis P, Roest S (1983) Phenotypic variation and ploidy level of plants regenerated from protoplasts of tetraploid potato (Solanum tuberosum L. cv. ‘Bintje’). Theor Appl Genet 65:329–338
Schieder O (1978) Somatic hybrids of Datura inoxia Mill + Datura discolor Bernh. and of Datura inoxia L. + Datura stramonium L. var ‘tatula’. 1. Selection and characterization. Mol Gen Genet 162:113–119
Shepard JF (1980) Mutant selection and plant regeneration from potato mesophyll protoplasts. In: Rubenstein I, Gengenbach B, Phillips RL, Green CE (eds) Genetic improvement of crops: emergent techniques. University of Minnesota press, Minneapolis, pp 185–219
Shepard JF, Bidney D, Barsby T, Kemble R (1983) Genetic transfer in plants through interspecific protoplast fusion. Science 219:683–688
Wenzel G (1980) Protoplast techniques incorporated into applied breeding programs. In: Ferenczy L, Farkas GL (eds) Advances in protoplast research. Pergamon Press, Oxford, pp 327–340
Author information
Authors and Affiliations
Additional information
Communicated by H. F. Linskens
Rights and permissions
About this article
Cite this article
Austin, S., Baer, M., Ehlenfeldt, M. et al. Intra-specific fusions in Solanum tuberosum . Theoret. Appl. Genetics 71, 172–175 (1985). https://doi.org/10.1007/BF00252052
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00252052