Abstract
It is a common practice in diagnostic hematology to mark blood cells with radioactive tracers, such as 51Cr, 55Fe, 75Fe, etc., to determine their lifespan and study their metabolism.
A program has been started to verify the possibility of marking blood cells with stable indicators, assayed by radioisotope-induced X-ray fluorescence analysis. All elements may be considered as indicators aggregating to blood cells, and for in vivo measurements, which are not toxic in the quantities injected, calculated on the basis of the limit of detection of the technique.
In this study platelets were marked with a very small quantity of stable compounds of rubidium, a potassium analog. Measurements were carried out in vitro on platelets of normal subjects and in vivo on platelets of rats and rabbits. Several survival curves were deduced and life-span values obtained in accord with previously reported values.
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Born, G.V.R., Cross, M.J.J.: The aggregation of blood platelets. J. Physiol. (Lond.) 168, 178 (1963)
Cesareo, R., Tallarida, G., Baldoni, F.: Determination of hemodynamic parameters in the rabbit by X-ray fluorescence excitation. Int. J. Appl. Radiat. Isot. 26, 285–289 (1975)
Cesareo, R., Del Principe, D., Mancuso, G., Tallarida, B.M.: In vitro labelling of platelets with stable Selenocystine. Int. J. Appl. Radiat. Isot. 27, 324–326 (1976)
Cesareo, R., Frazzoli, F.V., Sciuti, S.: Sensitivity of radioisotope XRF technique with particular reference to portable units. J. Radioan. Chem. 34, 157–170 (1976a)
Cesareo, R., Ciancarelli, G., Pacitti, C., Scarnati, E.: Labeling of blood platelets in rabbits with stable rubidium chloride, (submitted to Blood)
Kaufman, L.: Clinical use of stable indicators assayed in vitro by fluorescence excitation analysis. Trans. Am. Nucl. Soc. 21 (Suppl. 2), 62 (1975)
Morgan, M.C., Keating, R.P., Reisner, E.H.: Survival of radiochromate-labeled platelets in rabbits. J. Lab. Clin. Med. 46, 521–530 (1955)
Mürer, E.H.: Release reaction and energy metabolism in blood platelets, with special reference to the burst in oxygen uptake. Biochem. Biophys. Acta 320, 162 (1968)
Price, D.C.: The measurements of circulatory red cell volume using non-radioactive cesium and fluorescent excitation analysis, J. Lab. Clin. Med. 87, 535–543 (1976)
Wright, H.P.: The adhesiveness of blood platelets in normal subjects with varying concentration of anti-coagulants. J. Pathol. Bacteriol. 53, 255 (1941)
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Cesareo, R., Del Principe, D., Tallarida, B. et al. In vitro labeling of platelets with stable rubidium compounds. Eur J Nucl Med 3, 91–93 (1978). https://doi.org/10.1007/BF00251630
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DOI: https://doi.org/10.1007/BF00251630