Summary
RNA isolated from both rat phaeochromocytoma (PC12) cells and bovine adrenal medulla was size-selected and injected into oocytes of Xenopus laevis. Expression of the neuronal noradrenaline transporter was assayed 3 days after injection by measuring the nisoxetine-sensitive noradrenaline uptake. A 2 kilobase fraction of either total RNA derived from both tissues or of polyadenylated mRNA obtained from bovine adrenal medulla caused Na+-dependent transport of 3H-noradrenaline in the injected oocytes.
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Coppeneur, D., Lingen, B., Sanders, G. et al. Expression of the neuronal noradrenaline transporter in Xenopus laevis oocytes. Naunyn-Schmiedeberg's Arch Pharmacol 343, 334–336 (1991). https://doi.org/10.1007/BF00251136
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DOI: https://doi.org/10.1007/BF00251136