Summary
The distribution of actin, myosin and tropomyosin in freshly isolated and short-term cultured rat hepatocytes was investigated by use of both rhodaminyl-phalloidin staining and immunofluorescence techniques. The cytoskeletal proteins were mainly located in distinct areas of the hepatocyte membrane, corresponding to their accumulation in the bile-canalicular region of liver tissue. In freshly prepared cells, these sections resembled sharp, angled or branched bands, similar to the pattern of hemicanaliculi. During incubation in a monolayer culture, these bands were transformed to circular formations. Simultaneously, enclosed bile-canalicular spaces between undissociated hepatocytes were visualized by staining of actin, myosin, and tropomyosin. The preservation of canalicular cytoskeletal structures in isolated hepatocytes is an indication of cellular polarity. Our findings suggest a uniform association of membrane-bound F-actin with myosin and tropomyosin.
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References
Berry MN, Friend DS (1969) High-yield preparation of isolated rat liver parenchymal cells. J Cell Biol 43:506–529
Chamley-Campbell J, Campbell GR, Gröschel-Stewart U, Burnstock G (1977) FITC-labeled antibody staining of tropomyosin containing fibrils in smooth, cardiac and skeletal muscle cells, prefusion myoblasts, fibroblasts, endothelial cells and 3T3 cells in culture. Cell Tissue Res 183:153–166
Drenckhahn D, Gröschel-Stewart U, Kendrick-Jones J, Scholey JM (1983) Antibody to thymus myosin: its immunological characterization and use for immunocytochemical localization of myosin in vertebrate nonmuscle cells. Eur J Cell Biol 30:100–111
Faulstich H, Trischmann H, Mayer D (1983) Preparation of tetramethylrhodaminyl-phalloidin and uptake of the toxin into short-term cultured hepatocytes by endocytosis. Exp Cell Res 144:73–82
Grisham JW, Nopanitaya W, Compagno J (1976) Scanning electron microscopy of the liver: a review of methods and results. In: Popper H, Schaffner F (eds) Progress in liver disease Vol. 5:1–23, Grune and Straton, New York
Heine WD, Altmann HW, Gröschel-Stewart U (1976) Nachweis und Lokalisation von Aktin und Myosin in Leberepithelien von Mensch und Ratte —Immunfluoreszenzuntersuchungen. Verh Dtsch Ges Path 60:321
Motta PM (1984) The three-dimensional microanatomy of the liver. Arch Histol Jpn 47:1–30
Trevisan A, Gudat F, Guggenheim R, Krey G, Lüönd G, Stalder GA, Tondelli P, Bianchi L (1983) Electron microscopy of isolated human hepatocytes. La Ricerca Clin Lab 13:307–320
Vonk RJ, Jekel PA, Meijer DKF, Hardonk MJ (1977) Transport of drugs in isolated hepatocytes —the influence of bile salts. Biochem Pharmacol 27:397–405
Wehland J, Weber K (1980) Distribution of fluorescently labeled actin and tropomyosin after microinjection in living tissue culture cells, as observed with TV image intensification. Exp Cell Res 127:397–408
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Nickola, I., Frimmer, M. Preservation of cellular polarity in isolated hepatocytes. Cell Tissue Res. 243, 437–440 (1986). https://doi.org/10.1007/BF00251061
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DOI: https://doi.org/10.1007/BF00251061