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The adrenal: A new target organ of the calciotropic hormone 1,25-dihydroxyvitamin D3

Cell and Tissue Research volume 243, pages 299–302 (1986)Cite this article

Summary

Target cells for 1,25-dihydroxyvitamin D3 were demonstrated in the adrenal medulla by frozen-section autoradiography. The appearance of these target cells was age-dependent in neonatal mice. Immunocytochemical staining for phenylethanolamine-N-methyltransferase revealed that both epinephrine and non-epinephrine cells concentrate 1,25-dihydroxyvitamin D3 in their nuclei. In contrast, immunocytochemical staining for “vitamin D-dependent calcium-binding protein” (D-CaBP) demonstrated that D-CaBP immunoreactivity is localized in only a small percentage of adrenomedullary cells, in mice and rats. Comparison of PNMT and D-CaBP immunoreactivities in sequential sections showed that epinephrine-producing cells do not contain D-CaBP. These results indicate that adrenal medullary cells have receptors for 1,25-dihydroxyvitamin D3 and that 1,25-dihydroxyvitamin D3 may directly affect certain functions of these endocrine cells.

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Affiliations

  1. Department of Anatomy, University of North Carolina, Chapel Hill, NC, USA

    Samuel A. Clark, Walter E. Stumpf, Charles W. Bishop, Hector F. DeLuca, Dong H. Park & Tong H. Joh

  2. Department of Biochemistry, University of Wisconsin, Madison, WI, USA

    Charles W. Bishop & Hector F. DeLuca

  3. Laboratory of Neurobiology, Cornell University Medical College, New York, NY, USA

    Dong H. Park & Tong H. Joh

Authors
  1. Samuel A. Clark
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  2. Walter E. Stumpf
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  3. Charles W. Bishop
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  4. Hector F. DeLuca
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  5. Dong H. Park
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  6. Tong H. Joh
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Additional information

Supported by US PHS grant PCM8200569 and postdoctoral training grant AM07240-02

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Clark, S.A., Stumpf, W.E., Bishop, C.W. et al. The adrenal: A new target organ of the calciotropic hormone 1,25-dihydroxyvitamin D3 . Cell Tissue Res. 243, 299–302 (1986). https://doi.org/10.1007/BF00251043

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  • DOI: https://doi.org/10.1007/BF00251043

Key words

  • 1,25-dihydroxyvitamin D3
  • Adrenal medulla
  • Ontogenetic development
  • Phenylethanolamine-N-methyltransferase
  • Vitamin D-dependent calcium-binding protein
  • Mouse, rat