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Hierarchies in the binding of human factor XIII, factor XIIIa, and endothelial cell transglutaminase to human plasma fibrinogen, fibrin, and fibronectin

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Abstract

The affinities of Factor XIII (FXIII), Factor XIIIa (FXIIla), and cellular transglutaminase (Tg) for fibrinogen (Fgn), fibrin (Fbn), and fibronectin (Fn) were compared using a solid-phase binding assay. Initial rates of binding were as follows: FXIII bound Fbn 3-fold more than Fgn. FXIII did not bind Fn till 20 min. Increasing the ligands concentrations and binding time, resulted in weak binding of FXIII to Fn. FXIIla bound Fbn 2-fold more than Fgn and 28-fold more than Fn. Tg bound Fn ∼ 130-fold more than either Fgn or Fbn. At equilibrium, the extent of binding was determined to be as follows: FXIII bound Fbn 3–15-fold more than Fgn and 8-fold more than Fn. FXIIIa bound Fgn and Fbn equally and 12–25-fold more than Fn. FXIIla bound Fgn or Fbn 2-fold and 25-fold greater than FXIII-Fbn and FXIII-Fgn interactions, respectively. Tg bound about equally to Fgn and Fbn and 10–20-fold less than Fn. The K d s′ for FXIIla binding to Fn, Fgn, and Fbn were 100, 23, and 19 nM, respectively. The K d for Tg binding to Fn was 6.5 nM. The binding hierarchies are: [Tg-Fn]>[FXIIIa-Fgn]=[FXIIIa-Fbn]>[FXIII-Fbn]>[FXIIIFgn]=[FXIIIa-Fn]>[Tg-Fbn]=[Tg-Fgn]>[FXIII-Fn]. Such hierarchies could regulate the cross-linkings by FXIIIa and Tg during hemostasis, wound healing, and cell adhesion.

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Abbreviations

Tg:

cellular transglutaminase

FXIII:

coagulation factor XIII

FXIIla:

factor XIIIa (thrombin-activated FXIII)

Fgn:

human plasma fibrinogen

Fn:

human plasma fibronectin

Fbn:

human plasma fibrin (thrombin-cleaved fibrinogen)

ECM:

extracellular matrix

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Authors and Affiliations

  1. Oklahoma Medical Research Foundation, ZymeTx, Inc., 73104, Oklahoma City, OK

    Komandoor E. Achyuthan

  2. Departments of Urology, Oklahoma University Health Sciences Center, 73104, Oklahoma City, OK

    Tami C. Rowland & Paul J. Birckbichler

  3. Departments of Biochemistry & Molecular Biology, Oklahoma University Health Sciences Center, 73104, Oklahoma City, OK

    Ann M. Achyuthan

  4. Departments of Medicine, Oklahoma University Health Sciences Center, 73104, Oklahoma City, OK

    Kyung N. Lee

  5. Zymogenetics Inc., 98102, Seattle, WA, USA

    Paul D. Bishop

Authors
  1. Komandoor E. Achyuthan
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  2. Tami C. Rowland
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  3. Paul J. Birckbichler
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  4. Kyung N. Lee
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  5. Paul D. Bishop
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  6. Ann M. Achyuthan
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Achyuthan, K.E., Rowland, T.C., Birckbichler, P.J. et al. Hierarchies in the binding of human factor XIII, factor XIIIa, and endothelial cell transglutaminase to human plasma fibrinogen, fibrin, and fibronectin. Mol Cell Biochem 162, 43–49 (1996). https://doi.org/10.1007/BF00250994

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  • DOI: https://doi.org/10.1007/BF00250994

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