Summary
A new method for continuous blood collection using the Biostator is described. Blood is withdrawn through the double lumen catheter by a tube installed in the optional channel of the infusion pump. The amount of blood withdrawn from the patient is slightly greater than that necessary for continuous glucose analysis; the excess blood can be collected into assay tubes. Blood collection is continuous and produces a sample of diluted heparinized blood. The volume of blood collected depends on the size of the tube used, i.e. for a tube with a lumen diameter of 0.020 inches, the mean (±SD) volume collected was 1.21 ±0.07 ml/10 min (n = 13). The mean time interval between sampling and arrival at the glucose sensor by the double lumen catheter was 119 versus 108 s with the conventional method. The proposed modification does not affect blood glucose measurements (correlation coefficient compared with the reference method r = 0.9572; n = 13). To compensate for blood dilution, a dilution-factor depending on tubing diameter has to be calculated in each experiment.
Article PDF
Avoid common mistakes on your manuscript.
References
Hepp KD, Kerner W, Pfeiffer EF (Eds) (1979) Feedback-controlled and preprogrammed insulin infusion in diabetes mellitus. Horm Metab Res Suppl. Series 8
Verdonk CA, Rizza RA, Westland RE, Nelson RL, Gerich JE, Service FJ (1980) Glucose clamping using the Biostator GCIIS. Horm Metab Res 12: 133–135
Home PD, Massi-Benedetti M, Shepherd GAA, Hanning I, Alberti KGMM, Owens DR (1982) A comparison of the activity and disposal of semi-synthetic human insulin and porcine insulin in normal man by the glucose clamp technique. Diabetologia 22: 41–45
Valenta LJ, Elias AN, Grossman M, Tanner SM, Friis R (1982) A new test of peripheral insulin sensitivity in vivo using artificial beta cell. Life Sci 30: 867–873
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Castillo, M.J., Scheen, A.J., Luyckx, A.S. et al. Blood collection while using a continuous glucose analyzer without insertion of an additional venous catheter. Diabetologia 25, 120–122 (1983). https://doi.org/10.1007/BF00250899
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00250899