Summary
This paper describes the effect of different nutrients on the production of the macrolide polyene antibiotics, PA-5 and PA-7, produced by Streptoverticillium sp 43/16. Optimal production yields of PA-5 and PA-7 have been achieved with l-proline and glycine as nitrogen sources, respectively. Elsewhere, the presence of manganese as a metallic ion stimulated the antibiotic production significantly. The requirements of phosphate for optimal yields of both antibiotics (50 mM) are much higher than those described for strains of Streptomyces, which produce other macrolide polyene antibiotics. Magnesium is essential for this production. The specific production of PA-5 and PA-7 increases with concentrations of glucose up to 40 mM, but is inhibited at higher concentrations. It was found that the presence of ammonium salts and the amino acids l-cysteine and/or l-valine as nitrogen sources has negative effects on the production of both antibiotics.
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Soliveri, J., Mendoza, A. & Arias, ME. Effect of different nutrients on the production of polyene antibiotics PA-5 and PA-7 by Streptoverticillium sp 43/16 in chemically defined media. Appl Microbiol Biotechnol 28, 254–257 (1988). https://doi.org/10.1007/BF00250450
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DOI: https://doi.org/10.1007/BF00250450