Abstract
The effects of regression of cardiac hypertrophy on myocardial contractility and ventricular myosin isoenzymes were investigated in rats with renovascular hypertension. Six-week-old male Wistar rats were made hypertensive by constriction of one renal artery with a silver clip. Regression of cardiac hypertrophy was induced following the lowering of blood pressure by nephrectomy on the affected side 5–6 weeks after constriction of the renal artery and was maintained for 5–6 weeks. In contrast, myocardial hypertrophy was induced by 10–11 weeks of the hypertensive state. Isometric developed tension of isolated left ventricular papillary muscles was measured, while they were being perfused with Tyrode solution. Left ventricular myosin isoenzymes were separated by pyrophosphate gel electrophoresis. The ventricular to body weight ratio of the nephrectomized group was significantly lower than that of the hypertensive group, although it was greater than that of age-matched normal control rats. There were no significant differences in the isometric developed tension among three groups, the nephrectomized, hypertensive, and normal control rats. However, dT/dtmax tended to decrease in the hypertensive rats and recovered to normal in the nephrectomized rats. The left ventricular myosin isoenzyme pattern was shifted toward VM-3 in hypertensive rats and was shifted back toward VM-1 again in nephrectomized rats.
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Lompre AM, Schwartz K, d'Albis A, Lacombe G, Thiem NV, Swynghedauw B: Myosin isoenzyme redistribution in chronic heart overload. Nature 282: 105–107, 1979
Mercadier JJ, Lompre AM, Wisnewsky C, Samuel JL, Bercovici J, Swynghedauw B, Schwartz K: Myosin isoenzymic changes in several models of rat cardiac hypertrophy. Cir Res 49: 525–532, 1981
Rupp H: The adaptive changes in the isoenzyme pattern of myosin from hypertrophied rat myocardium as a result of pressure overload and physical training. Basic Res Cardiol 76: 79–88, 1981
Alpert NR, Mulieri LA: Increased myothermal economy of isometric force generation in compensated cardiac hypertrophy induced by pulmonary artery constriction in the rabbit. Circ Res 50: 491–500, 1982
Holubarsch Ch, Litten RZ, Mulieri LA, Alpert NR: Energetic changes of myocardium as an adaptation to chronic hemodynamic overload and thyroid gland activity. Basic Res Cardiol 80: 582–593, 1985
Jacob R, Kissling G, Ebrecht G, Holubarsch Ch, Medugorac I, Rupp H: Adaptive and pathological alterations in experimental cardiac hypertrophy. In: E Chazov, V Saks and G Rona (eds) Advances in Myocardiology. Plenum Press, New York, 1983, pp 55–77
Kissling G, Rupp H, Malloy L, Jacob R: Alteration in cardiac oxygen consumption under chronic pressure overload. Significance of the isoenzyme pattern of myosin. Basic Res Cardiol 77: 255–269, 1982
d'Albis A, Pantaloni C, Becher JJ: An electrophoretic study of native myosin isoenzymes and of their subunit content. Eur J Biochem 99: 261–272, 1979
Hoh JY, McGrath PA, Hale PT: Electrophoretic analysis of multiple forms of rat cardiac myosin: effects of hypophysectomy and thyroxin replacement. J Mol Cell Cardiol 10: 1053–1076, 1978
Rupp H, Jacob R: Response of blood pressure and cardiac myosin polymorphism to swimming training in the spontaneously hypertensive rat. Can J Physiol Pharmacol 60: 1098–1103, 1982.
Otsuka LY, Carretero OA, Albertini R, Binia A: Angiotensin and sodium balance: their role in chronic two-kidney Goldblatt hypertension. Hypertension 1: 389–396, 1979
Ten Berg RGM, Leenen FHH, De Jong W: Plasma renin activity and sodium, potassium and water excretion during reversal of hypertension in the one-clip, two-kidney hypertensive rat. Clin Sci 57: 47–52, 1979
Oates HF, Stokes GS, Storey BG: Plasma renin concentration in hypertension produced by unilateral renal artery constriction in the rat. Clin Exp Pharmacol Physiol 2: 289–296, 1975
Thurston H, Bing RF, Swales JD: Reversal of two-kidney, oneclip hypertension in the rat. Hypertension 2: 256–265, 1980
Dargie HJ, Franklin SS, Reid JL: Central and peripheral noradrenaline in the two-kidney model of renovascular hypertension in the rat. Br J Pharmacol 61: 213–215, 1977
Edmunds ME, Russell GI, Bing RF: Reversal of experimental renovascular hypertension. J Hypertens 9: 289–301, 1991
Simpson P: Norepinephrine-stimulated hypertrophy of cultured rat myocardial cells in an alpha 1 adrenergic response. J Clin Invest 72: 732–738, 1983
Xenophontos XP, Watson PA, Chua BHL, Haneda T, Morgan HE: Increased cyclic AMP content accelerates protein synthesis in rat heart. Circ Res 65: 647–656, 1989
Zierhut W, Zimmer HG: Significance of myocardial alpha- and beta-adrenoceptors in catecholamine-induced cardiac hypertrophy. Circ Res 65: 1417–1425, 1989
Aceto JF, Baker KM: [Sar 1] angiotensin II receptor-mediated stimulation of protein synthesis in chick heart cells. Am J Physiol 258: H806-H813, 1990
Baker KM, Aceto JF: Angiotensin II stimulation of protein synthesis and cell growth in chick heart cells. Am J Physiol 259: H610-H618, 1990
Honda M, Morioka S, Yamada S, Ohoka M, Tanabe K, Matsuno Y, Murakami Y, Kobayashi S, Ishinaga Y, Moriyama K: Study of cardiac hypertrophy-humoral factors that stimulate protein metabolism of cultured rat heart cells. Jpn Circ J 53: 795–806, 1989
Nagano M, Ohkubo T, Suzuki H: Growth factor and its role for cardiac hypertrophy. In: R Jacob, L Seipel, IH Zucker (eds) Cardiac Dilatation. Gustav Fischer Verlag, Stuttgart, 1990, pp 81–88
Komuro I, Kurabayashi M, Shibazaki Y, Katoh Y, Hoh E, Kaida T, Ikeda K, Takaku F, Yazaki Y: Molecular mechanism of cardiac hypertrophy. Jpn Circ J 54: 526–534, 1990
Mann DL, Kent RL, Cooper G: Load regulation of the properties of adult feline cardiocytes: growth induction by cellular deformation. Circ Res 64: 1079–1090, 1989
Takeda N, Dominiak T, Türck D, Rupp J, Jacob R: The influence of endurance training on mechanical catecholamine responsiveness, beta-adrenoceptor density and myosin isoenzyme pattern of rat ventricular myocardium. Basic Res Cardiol 80: 88–99, 1985
Rupp H, Bukhari AR, Jacob R: Regulation of cardiac myosin isoenzymes — the interrelationship with catecholamine metabolism (Abstr). J Mol Cell Cardiol 15 (Suppl 1): 317, 1983
Takeda N: Effects of thyroid hormones on myocardial contractility and ventricular myosin isoenzymes. Jikeikai Med J 37 (Suppl): 45–52, 1990
Takeda N, Ohkubo T, Hatanaka T, Takeda A, Nakamura I, Nagano M: Myocardial contractility and left ventricular myosin isoenzyme pattern in cardiac hypertrophy due to chronic volume overload. Basic Res Cardiol 82 (Suppl 2): 215–221, 1987
Dillmann WH: Diabetes mellitus induces changes in cardiac myosin of the rat. Diabetes 29: 579–582, 1980
Takeda N, Nakamura I, Hatanaka T, Ohkubo T, Nagano M: Myocardial mechanical and myosin isoenzyme alterations in streptozotocin-diabetic rats. Jpn Heart J 29: 455–463, 1988
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Iwai, T., Takeda, N., Tuchiya, M. et al. Effects of regression of cardiac hypertrophy on myocardial contractility and ventricular myosin isoenzymes. Mol Cell Biochem 118, 99–103 (1992). https://doi.org/10.1007/BF00249699
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DOI: https://doi.org/10.1007/BF00249699