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Effect of Cu2+-ascorbic acid on lipid peroxidation, Mg2+-ATPase activity and spectrin of RBC membrane and reversal by erythropoietin

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Abstract

The effect of erythropoietin (Ep), a glycoprotein hormone, has been studied on lipid peroxidation induced by Cu2+ and ascorbate in vitro, Mg2+ ATPase activity and spectrin of RBC membrane. Our present investigation reveals that Cu2+ and ascorbic acid increases lipid peroxidation of RBC membrane significantly. It has further been observed that under the same experimental condition spectrin, a major cytoskeleton membrane protein, and Mg2+-ATPase activity of RBC membrane decrease significantly. However, exogenous administration of Ep completely restores lipid peroxidation and Mg2+-ATPase activity and partially recovers spectrin of RBC membrane.

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Authors and Affiliations

  1. Guha Institute of Biochemistry, 5515, Purna Das Road, 700029, Calcutta, India

    Aindrila Chattopadhyay & Tapasi Das Choudhury

  2. Indian Institute of Chemical Biology, 4 Raja S. C. Mullick Road, 700032, Calcutta, India

    Mukul K. Basu & Asoke G. Datta

Authors
  1. Aindrila Chattopadhyay
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  2. Tapasi Das Choudhury
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  3. Mukul K. Basu
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  4. Asoke G. Datta
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Chattopadhyay, A., Choudhury, T.D., Basu, M.K. et al. Effect of Cu2+-ascorbic acid on lipid peroxidation, Mg2+-ATPase activity and spectrin of RBC membrane and reversal by erythropoietin. Mol Cell Biochem 118, 23–30 (1992). https://doi.org/10.1007/BF00249691

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  • DOI: https://doi.org/10.1007/BF00249691

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