Skip to main content

Hypothalamic lesions stimulating growth hormone cell activity in the goldfish

Cell and Tissue Research volume 214, pages 387–395 (1981)Cite this article

Summary

The cytology of the growth-hormone (GH) cells of the goldfish pituitary were examined following electrothermic lesions of the anterior praeoptic hypothalamus and telencephalon. Following lesions of the nucleus preopticus (NPO) light microscopy of the pituitary revealed a significant increase in the nuclear diameter and a degranulation of the GH cells. Lesions of the telencephalon anterior or dorsal to the NPO had no cytological effect on the GH cells. The ultrastructural appearance of the GH cells of NPO-lesioned fish was characterized by a marked degranulation of the cytoplasm and a proliferation of the rough endoplasmic reticulum indicative of enhanced secretory activity. The GH cells of the proximal pars distalis (PPD) are directly innervated by peptidergic (type A) and aminergic-like (type B) neurosecretory axons. Following lesions of the NPO, there was a marked reduction in the number of type A fibers in the PPD. These results suggest that the type A fibers innervating the GH cells originate in the NPO and act to inhibit the secretory activity of the GH cells.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

References

  • Batten TFC, Ingleton PM, Ball JN (1979) Ultrastructural and formaldehyde-fluorescence studies on the hypothalamus of Poecilia latipinna (Teleostei, Cyprinodontiformes). Gen Comp Endocrinol 39:87–109

    Google Scholar 

  • Chihara K, Arimura A, Kubli-Garfias C, Schally A (1979) Enhancement of immunoreactive somatostatin release in hypophysial portal blood by electrical stimulation of the preoptic area in the rat. Endocrinology 105:1416–1418

    Google Scholar 

  • Crim JW, Dickoff WW, Gorbman A (1978) Comparative endocrinology of piscine hypothalamic hypophysiotropic peptides: Discussion and activity. Am Zool 18:411:424

    Google Scholar 

  • Critchlow V, Rice RW, Abe K, Vale W (1978) Somatostatin content of the median eminence in female rats with lesion-induced disruption of the inhibitory control of growth hormone secretion. Endocrinology 103:817–825

    Google Scholar 

  • Dubois MP, Kolodziejczyk E (1975) Centre hypothalamiques du rat sécrétant la somatostatine: répartitions des péricaryons en 2 systèmes magnoet parvocellularis (étude immunocytologique). CR Acad Sci [D] (Paris) 281:1737–1740

    Google Scholar 

  • Dubois MP, Barry J, Leonardelli J (1974) Mise en évidence par immunofluorescence et répartition de la somatostatine (SRIF) dans l'éminence médiane des vertébrés (mamifères, oiseaux, amphibiens, poissons). CR Acad Sci [D] (Paris) 279:1899–1903

    Google Scholar 

  • Dubois MP, Billard R, Breton B, Peter RE (1979) Comparative distribution of somatostatin, LH-RH, neurophysin, and α-endorphin in the rainbow trout: An immunological study. Gen Comp Endocrinol 37:220–232

    Google Scholar 

  • Fryer JN, Peter RE (1977a) Hypothalamic control of ACTH secretion in goldfish. II. Hypothalamic lesioning studies. Gen Comp Endocrinol 33:204–214

    Google Scholar 

  • Fryer JN, Peter RE (1977b) Hypothalamic control of ACTH secretion in goldfish. III. Hypothalamic cortisol implant studies. Gen Comp Endocrinol 33:215–225

    Google Scholar 

  • Fryer JN, Nishioka RS, Bern HA (1979) Somatostatin inhibition of teleost growth hormone secretion. Gen Comp Endocrinol 38:244–246

    Google Scholar 

  • Goossens N, Dierickx K, Vandesande F (1977) Immunocytochemical localization of vasotocin and isotocin in the preoptico-hypophysial neurosecretory system of teleosts. Gen Comp Endocrinol 32:371–375

    Google Scholar 

  • Kaul S, Vollrath L (1974a) The goldfish pituitary. I. Cytology. Cell Tissue Res 154:211–230

    Google Scholar 

  • Kaul S, Vollrath L (1974b) The goldfish pituitary. II. Innervation. Cell Tissue Res 154:231–249

    Google Scholar 

  • King JA, Millar RP (1979) Phylogenetic and anatomical distribution of somatostatin in vertebrates. Endocrinology 105:1322–1329

    Google Scholar 

  • Knowles Sir F (1965) Evidence for a dual control, by neurosecretion, of hormone synthesis and hormone release in the pituitary of the Dogfish Scyliorchinus stellaris. Phil Trans 249:435–456

    Google Scholar 

  • Leatherland JF (1972) Histophysiology and innervation of the pituitary gland of the goldfish Carassius auratus L.: light and electron microscope investigation. Can J Zool 50:835–844

    Google Scholar 

  • Martin JB (1974) The role of hypothalamic and extrahypothalamic structures in the control of GH secretion. In: Raiti S (ed) Advances in Human Growth Hormone Research. NIH, Washington, DC

    Google Scholar 

  • Peter RE, Gill VE (1975) A stereotaxic atlas and technique for forebrain nuclei of the goldfish, Carassius auratus. J Comp Neurol 159:69–102

    Google Scholar 

  • Peter RE, Fryer JN (1980) Endocrine functions of the hypothalamus of actinopterygians. In: Davis RE, Northcutt RG (eds) Fish Neurobiology and Behavior. University of Michigan Press, Ann Arbor (in press)

    Google Scholar 

  • Reaves TA Jr, Hayward JN (1979a) Chemical identification of physiologically-defined, dye-injected neuroendocrine cells in the preoptic nucleus of goldfish hypothalamus. Endocrine Soc Abst 61:160

    Google Scholar 

  • Reaves TA Jr, Hayward JN (1979b) Immunochemical identification of enkephalinergic neurons in the hypothalamic magnocellular preoptic nucleus of the goldfish, Carassius auratus. Cell Tissue Res 200:147–151

    Google Scholar 

  • Terlou M, Ekengren B (1979) Nucleus praeopticus and nucleus lateralis tuberis of Salmo salar and Salmo gairdneri: Structure and relationship to the hypophysis. Cell Tissue Res 197:1–21

    Google Scholar 

  • Vale W, Ling N, Rivier J, Villareal J, Rivier C, Douglas C, Brown M (1976) Anatomic and phylogenetic distribution of somatostatin. Metabolism 25:1491–1494

    Google Scholar 

Download references

Author information

Affiliations

  1. Department of Anatomy, School of Medicine, Faculty of Health Sciences, University of Ottawa, K1N9A9, Ottawa, Canada

    J. N. Fryer

Authors
  1. J. N. Fryer
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Fryer, J.N. Hypothalamic lesions stimulating growth hormone cell activity in the goldfish. Cell Tissue Res. 214, 387–395 (1981). https://doi.org/10.1007/BF00249219

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00249219

Key words

  • Growth hormone (GH)
  • Somatotropin
  • Anterior pituitary
  • Nucleus preopticus (NPO)
  • Teleost