Summary
The penetration of horseradish peroxidase (HRP) from blood into ventricle via the avian choroid plexus was examined by electron microscopy in order to study the development of the blood-cerebrospinal fluid barrier (BCSFB). 1–20 mg HRP was injected into the allantoic vein of chick embryos between the 7th and 21st day of incubation. Until the 8th day (stage 34) the reaction products of injected HRP were observed in the interepithelial clefts at both luminal (ventricular) and abluminal sides. At the 9th day (stage 35), their penetration was blocked at most apical junctional complexes of the choroidal epithelia. At the 10th day (stage 36) and at every subsequent stage, HRP molecules were completely impeded at the apical tight junctions.
These results show that the BCSFB to HRP in the avian choroidal epithelium is completely established by the 10th day, when molecules of HRP are still able to permeate between the capillary endothelia in the cerebellum and the spinal cord (Wakai and Hirokawa 1978).
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References
Becker NH, Almazon R (1968) Evidence for the functional polarization of micropinocytotic vesicles in the rat choroid plexus. J Histochem Cytochem 16:278–279
Becker NH, Novikoff AB, Zimmerman HM (1967) Fine structure observations of the uptake of intravenously injected peroxidase by the rat choroid plexus. J Histochem Cytochem 15:160–165
Birge WJ, Rose AD, Haywood JR, Doolin PF (1974) Development of the blood-CSF barrier to proteins and differentiation of CSF in the chick embryo. Dev Biol 41:245–254
Bouldin TW, Krigman MR (1975) Differential permeability of cerebral capillary and choroid plexus to lanthanum ion. Brain Res 99:444–448
Brightman MW (1968) The intracerebral movement of proteins injected into blood and cerebrospinal fluid of mice. In: Lajtha A, Ford DH (eds) Prog Brain Res, Vol 29. Brain Barrier Systems. Amsterdam, Elsevier, pp 19–37
Brightman MW (1975) Ultrastructural characteristics of adult choroid plexus: Relation to the bloodcerebrospinal fluid barrier to proteins. In: Netsky MG, Shuangshati S (eds) The choroid plexus in health and disease. University Press of Virginia, Charlottesville, pp 86–112
Brightman MW, Reese TS (1969) Junctions between intimately apposed cell membranes in the vertebrate brain. J Cell Biol 40:648–677
Brightman MW, Reese TS, Feder N (1970) Assessment with the electron microscope of the permeability to peroxidase of cerebral endothelium and epithelium in mice and sharks. In: Crone C, Lassen NA (eds) The transfer of molecules and ions between capillary blood and tissue. Alfred Benzon Symposium II. Copenhagen, Munksgaard, pp 468–476
Carter LP, Beggs J, Waggener JD (1972) Ultrastructure of three choroid plexus papilloma. Cancer 30:1130–1136
Castel M, Sahar A, Erlij D (1974) The movement of lanthanum across diffusion barriers in the choroid plexus of the cat. Brain Res 67:178–184
Claude P, Goodenough DA (1973) Fracture faces of zonulae occludentes from “tight and leaky” epithelia. J Cell Biol 58:390–400
Davis DA, Milhorat TH (1975) The blood-brain barrier of the rat choroid plexus. Anat Rec 181:779–790
Delorme P, Gayet J, Grignon G (1970) Ultrastructural study on transcapillary exchanges in the developing telencephalon of the chicken. Brain Res 22:269–283
Dermietzel R, Meller K, Tetzlaff W, Waelsch M (1977) In vivo and in vitro formation of the junctional complex in choroid epithelium. Cell Tissue Res 181:427–441
Deurs B van, Moller M, Amtorp O (1978) Uptake of horseradish peroxidase from CSF into the choroid plexus of the rat, with special reference to transepithelial transport. Cell Tissue Res 187:215–234
Ghatak NR, McWhorter JM (1976) Ultrastructural evidence for CSF production by a choroid plexus papillomas. J Neurosurg 45:409–415
Hamburger V, Hamilton HL (1951) A series of normal stages in the development of the chick embryo. J Morphol 88:49–92
Luft JH (1971) Ruthenium red and violet. Anat Rec 171:347–442
Meller K, Wechsler W (1965) Elektronenmikroskopische Untersuchung der Entwicklung des telencephalen Plexus chorioideus des Huhnes. Z Zellforsch 65:420–444
Milhorat TH (1972) Hydrocephalus and the cerebrospinal fluid. Baltimore, Williams and Wilkins
Milhorat TH (1977) Structure and function of the choroid plexus and other sites of cerebrospinal fluid formation. Int Rev Cytol 47:225–288
Milhorat TH, Hammock MK, Fenstermacher JD (1971) Cerebrospinal fluid production by the choroid plexus and brain. Science 173:330–332
Milhorat TH, Davis DA, Lloyd BT Jr (1973) Two morphologically distinct blood-brain barriers preventing entry of cytochrome C into cerebrospinal fluid. Science 180:76–78
Milhorat TH, Davis DA, Hammock MK (1975) Experimental intracerebral movement of electron microscopic tracers of various molecular sizes. J Neurosurg 42:315–329
Milhorat TH, Davis DA, Hammock MK (1976) Choroid plexus papilloma. II. Ultrastructure and ultracytochemical localization of Na-K-ATPase. Child's Brain 2:290–303
Reese TS, Karnovsky MJ (1967) Fine structural localization of a blood-brain barrier to exogenous peroxidase. J Cell Biol 34:207–217
Richards JG (1978) Permeability of intercellular junctions in brain epithelia and endothelia to exogenous amine: Cytochemical localization of extracellular 5-hydroxydopamine. J Neurocytol 7:61–70
Romanoff AL (1967) Biochemistry of the avian embryo. New York, Interscience
Romanoff AL (1970) The avian embryo. Structural and functional development. Macmillan, New York
Śtastný F, Rychter Z (1976) Quantitative development of choroid plexus in chick embryo cerebral ventricles. Acta Neurol Scand 53:251–259
Wakai S, Hirokawa N (1978) Development of the blood-brain barrier to horseradish peroxidase in the chick embryo. Cell Tissue Res 195:195–203
Wakai S, Matsutani M, Mizutani H, Sano K (1979) Tight junctions in choroid plexus papillomas. Acta Neuropathol 45:159–160
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Wakai, S., Hirokawa, N. Development of blood-cerebrospinal fluid barrier to horseradish peroxidase in the avian choroidal epithelium. Cell Tissue Res. 214, 271–278 (1981). https://doi.org/10.1007/BF00249211
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DOI: https://doi.org/10.1007/BF00249211