Abstract
The pH in the cytoplasm of aerobic and anaerobic cells of the green algae Chlorella fusca and Chlorella vulgaris was determined in dependence on the pH of the external medium, which was varied between pH 3 and pH 10. In aerobic cells of both species the cytoplasmic pH is maintained at a value above 7.2 even at an external pH of 3 and below 7.8 at an external pH of 10. In anaerobic cells the cytoplasmic pH shows linear dependence on external pH in the range of pH 6 to 9 (cytoplasmic pH 6.9 to 7.2), while below an external pH of 6 cytoplasmic pH is maintained at about 6.5.
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Abbreviations
- CCCP:
-
Carbonylcyanide-m-chlorophenyl-hydrazone
- EDTA:
-
Ethylendiaminetetraacetic acid
- MES:
-
2-(N-Morpholino)-ethanesulfonic acid
- MOPSO:
-
3-(N-Morpholino)-2-hydroxy-propanesulfonic acid
- NMR:
-
Nuclear Magnetic Resonance
- pH cyt:
-
cytoplasmic pH
- pH ex:
-
external pH
- PIPES:
-
Piperazine-N,N′-bis(2-ethanesulfonic acid)
- PPi :
-
Pyrophosphate
- PP1, PP2, PP3 :
-
1st, 2nd, 3rd phosphate group of polyphosphates
- PP4 :
-
core phosphate groups of polyphosphates
- TRIS:
-
Tris-hydroxymethyl-aminomethane
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Küsel, A.C., Sianoudis, J., Leibfritz, D. et al. The dependence of the cytoplasmic pH in aerobic and anaerobic cells of the green algae Chlorella fusca and Chlorella vulgaris on the pH of the medium as determined by 31P in vivo NMR spectroscopy. Arch. Microbiol. 153, 254–258 (1990). https://doi.org/10.1007/BF00249077
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DOI: https://doi.org/10.1007/BF00249077