Abstract
We have studied the primary structures of myosins from chicken muscles in order to clarify the relationship between structure and function of muscle myosin. The primary structures of the various kinds of light chains from chicken muscle myosins have been determined. We also report the primary structure of the 23K fragment of subfragment-1 (S-1) component from the heavy chain of chicken fast skeletal muscle myosin. In addition, antibody was prepared against the 23K fragment. The antibody was found to inhibit the Mg2+-ATPase activity and the initial Pi burst of the ATPase in the S-1 component. The antibody suppressed the ATP-induced fluorescence enhancement of S-1, though it did not suppress the binding of ATP to S-1. These results are also discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Engelhardt W. A., and Ljubimova M. N. (1939). Nature 144, 668–669.
Huxley A. F., and Nidergerke R. (1954) Nature 173, 971–972.
Huxley H. E., and Hanson J. (1954). Nature 173, 973–976.
Maita T., Umegane T., Kato Y., and Matsuda G. (1980). Eur. J. Biochem. 107, 565–575.
Maita T., Chen J.-I., and Matsuda G. (1981a). Eur. J. Biochem. 117, 417–424.
Maita T., Umegane T., and Matsuda G. (1981b). Eur. J. Biochem. 114, 45–49.
Matsuda G. (1983). Adv. Biophys. 16, 185–218.
Matsuda G., Suzuyama Y., Maita T., and Umegane T. (1977). FEBS Lett. 84, 53–56.
Matsuda G., Maita T., Kato Y., Chen J.-I., and Umegane T. (1981a). FEBS Lett. 135, 232–236.
Matsuda G., Maita T., Umegane T. (1981b). FEBS Lett. 126, 111–113.
Miyanishi T., Saku T., and Matsuda G. (1984). J. Biochem. 95, 1823–1826.
Szent-Gyorgi A. (1947). Chemistry of Muscular Contraction, Academic Press, New York.
Tong S. W., and Elzinga M. (1983). J. Biol. Chem. 258, 13100–13110.
Tonomura Y. (1972). Muscle Proteins, Muscle Contraction and Cation Transport, University of Tokyo Press, Tokyo.
Author information
Authors and Affiliations
Additional information
This article was presented during the proceedings of the International Conference on Macromolecular Structure and Function, held at the National Defence Medical College, Tokorozawa, Japan, December 1985.
Rights and permissions
About this article
Cite this article
Matsuda, G., Maita, T., Miyanishi, T. et al. Structure and function of muscle myosin. J Protein Chem 6, 33–46 (1987). https://doi.org/10.1007/BF00248825
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF00248825