Summary
The coexistence of immunoreactivities for glutamic acid decarboxylase (GAD), tyrosine hydroxylase (TH) and substance P (SP) was revealed in the hamster main olfactory bulb, using the peroxidase-antiperoxidase immunohistochemical method. Adjacent 40 μm thick Vibratome sections were incubated in different antisera and those cells which were bisected by the plane of sectioning were identified at the paired surfaces of two consecutive sections. The coexistence of the immunoreactivities for 1) TH and GAD, 2) TH and SP and 3) GAD and SP in the same cells could thus be determined by observing the immunoreactivity of the two halves of the cell incubated in two different antisera. About 70% of TH-like immunoreactive (TH-LI) neurons in the periglomerular region also contained GAD-like immunoreactivity, whereas about 45% of GAD-LI ones were also TH-like immunoreactive. Furthermore, almost all (more than 95%) of SP-LI neurons contained both GAD-like and TH-like immunoreactivities. These observations indicate that in the periglomerular region of the hamster main olfactory bulb, some neurons (about 9% of all neurons containing TH-like and/or GAD-like immunoreactivities) may contain three different categories of neuroactive substances, that is, amino acid (GABA), amine (dopamine) and peptide (SP).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Baker H (1986a) Species differences in the distribution of substance P and tyrosine hydroxylase immunoreactivity in the olfactory bulb. J Comp Neurol 252: 206–226
Baker H (1986b) Substance P and tyrosine hydroxylase are localized in different neurons of the hamster olfactory bulb. Exp Brain Res 65: 245–249
Baker H, Kawano T, Margolis FL, Joh TH (1983) Transneuronal regulation of tyrosine hydroxylase expression in olfactory bulb of mouse and rat. J Neurosci 3: 69–78
Black IB, Adler JE, Dreyfus CF, Friedman WF, LaGamma EF, Roach AH (1987) Biochemistry of information storage in the nervous system. Science 236: 1263–1268
Brunjes PC, Smith-Crafts L, McCarty R (1985) Unilateral odor deprivation: effects on the development of olfactory bulb catecholamines and behavior. Dev Brain Res 22: 1–6
Burd GD, Davis BJ, Macrides F (1982) Ultrastructural identification of substance P immunoreactive neurons in the main olfactory bulb of the hamster. Neuroscience 7: 2697–2704
Chan-Palay V, Jonsson G, Palay SL (1978) Serotonin and substance P coexist in neurons of the rat's central nervous system. Proc Natl Acad Sci USA 75: 1582–1586
Davis BJ, Macrides F (1983) Tyrosine hydroxylase immunoreactive neurons and fibers in the olfactory system of the hamster. J Com Neurol 214: 427–440
Davis BJ, Burd GD, Macrides F (1982) Localization of methionine-enkephalin, substance P, and somatostatin immunoreactivities in the main olfactory bulb of the hamster. J Comp Neurol 204: 377–383
Eckenstein F, Baughman RW (1984) Two types of cholinergic innervation in cortex, one co-localized with vasoactive intestinal polypeptide. Nature 309: 153–155
Halász N, Shepherd GM (1983) Neurochemistry of the vertebrate olfactory bulb. Neuroscience 10: 579–619
Halász N, Johansson O, Hökfelt T, Ljungdahl Å, Goldstein M (1981) Immunohistochemical identification of two types of dopamine neuron in the rat olfactory bulb as seen by serial sectioning. J Neurocytol 10: 251–259
Halász N, Ljungdahl Å, Hökfelt T, Johansson O, Goldstein M, Park D, Biberfeld P (1977) Transmitter histochemistry of the rat olfactory bulb. I. Immunohistochemical localization of monoamine synthesizing enzymes. Support for intrabulbar, periglomerular dopamine neurons. Brain Res 126: 455–474
Hökfelt T, Ljungdahl Å, Steinbusch H, Verhofstad A, Nilsson G, Brodin E, Pernow B, Goldstein M (1978) Immunohistochemical evidence of substance P-like immunoreactivity in some 5-hydroxytryptamine-containing neurons in the rat central nervous system. Neuroscience 3: 517–538
Hökfelt T, Holets VR, Staines W, Meister B, Melander T, Schalling M, Schultzberg M, Freedman J, Bjöklund H, Olson L, Lindh B, Elfvin L-G, Lundberg JM, Lindgsen JA, Sammuelsson B, Pernow B, Terenius L, Post C, Everitt B, Goldstein M (1986) Coexistence of neuronal messengers — an overview. Prog Brain Res 68: 33–70
Johansson O, Hökfelt T, Pernow B, Jeffcoate SL, White N, Steinbusch HWM, Verhofstad AAJ, Emson PC, Spindel E (1981) Immunohistochemical support for three putative transmitters in one neuron: coexistence of 5-hydroxytryptamine, substance P- and thyrotropin releasing hormone-like immunoreactivity in medullary neurons projecting to the spinal cord. Neuroscience 6: 1857–1881
Joseph SA, Sternberger LA (1979) The unlabeled antibody method. Contrasting color staining of β-lipotropin and ACTH-associated hypothalamic peptides without antibody removal. J Histochem Cytochem 27: 1430–1437
Kawano T, Margolis FL (1982) Transsynaptic regulation of olfactory bulb catecholamines in mice and rats. J Neurochem 39: 342–348
Kosaka T, Hataguchi Y, Hama K, Nagatsu I, Wu J-Y (1985a) Coexistence of immunoreactivities for glutamate decarboxylase and tyrosine hydroxylase in some neurons in the periglomerular region of the rat main olfactory bulb: possible coexistence of gamma-aminobutyric acid (GABA) and dopamine. Brain Res 343: 166–171
Kosaka T, Kosaka K, Tateishi K, Hamaoka Y, Yanaihara N, Wu J-Y, Hama K (1985b) GABAergic neurons containing CCK-8-like and/or VIP-like immunoreactivities in the rat hippocampus and dentate gyrus. J Comp Neurol 239: 420–430
Kosaka K, Hama K, Nagatsu I, Wu J-Y, Ottersen O, Storm-Mathisen J, Kosaka T (1987a) Postnatal development of neurons containing both catecholaminergic and GABAergic traits in the rat main olfactory bulb. Brain Res 403: 355–360
Kosaka T, Kosaka K, Hataguchi Y, Nagatsu I, Wu J-Y, Ottersen OP, Storm-Mathisen J, Hama K (1987b) Catecholaminergic neurons containing GABA-like and/or glutamic acid decarboxylase-like immunoreactivities in various brain regions of the rat. Exp Brain Res 66: 191–210
Kosaka T, Kosaka K, Heizmann CW, Nagatsu I, Wu J-Y, Yanaihara N, Hama K (1987c) An aspect of the organization of the GABAergic system in the rat main olfactory bulb: laminar distribution of immunohistochemically defined subpopulations of GABAergic neurons. Brain Res 411: 373–378
Kosaka T, Kosaka K, Hama K, Wu J-Y, Nagatsu I (1987d) Differential effect of functional olfactory deprivation on the GABAergic and catecholaminergic traits in the rat main olfactory bulb. Brain Res 413: 197–203
Kosaka T, Tauchi M, Dahl JL (1988) Cholinergic neurons containing GABA-like and/or glutamic acid decarboxylaselike immunoreactivities in various brain regions of the rat. Exp Brain Res (in press)
Kream RM, Davis BJ, Kawano T, Margolis F, Macrides F (1984) Sustance P and catecholaminergic expression in neurons of the hamster main olfactory bulb. J Comp Neurol 222: 140–154
Millhorn DE, Hökfelt T, Seroogy K, Oertel W, Verhofstad AAJ, Wu J-Y (1987) Immunohistochemical evidence for colocalization of γ-aminobutyric acid and serotonin in neurons of the ventral medulla oblongata projecting to the spinal cord. Brain Res 410: 179–185
Nagatsu I (1983) Immunohistochemistry of biogenic amines and immunoenzyme-histochemistry of catecholamine-synthesizing enzymes. In: Parvez S, Nagatsu T, Nagatsu I, Parvez H (eds) Methods in biogenic amine research. Elsevier/North-Holland, Amsterdam, pp 873–909
Oertel WH, Graybiel AN, Mugnaini E, Elde RP, Schmechel DE, Kopin IJ (1983) Coexistence of glutamic acid decarboxylaseand somatostatin-like immunoreactivity in neurons of the feline nucleus reticularis thalami. J Neurosci 3: 1322–1332
Sternberger LA (1979) Immunocytochemistry, 2nd edn. Wiley, New York
Sternberger LA, Joseph SA (1979) The unlabeled antibody method. Contrasting color staining of paired pituitary hormones without antibody removal. J Histochem Cytochem 11: 1424–1429
Wilson CJ, Groves PM (1979) A simple and rapid section embedding technique for sequential light and electron microscopic examination of individually stained central neurons. J Neurosci Methods 1: 383–391
Wu J-Y, Lin C-T, Brandon C, Chan TS, Möhler CH, Richards JG (1982) Regulation and immunocytochemical characterization of glutamic acid decarboxylase. In: Palay S, Chan-Palay V (eds) Cytochemical methods in neuroanatomy. Alan R. Liss, New York, pp 279–296
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kosaka, K., Hama, K., Nagatsu, I. et al. Possible coexistence of amino acid (γ-aminobutyric acid), amine (dopamine) and peptide (substance P); neurons containing immunoreactivities for glutamic acid decarboxylase, tyrosine hydroxylase and substance P in the hamster main olfactory bulb. Exp Brain Res 71, 633–642 (1988). https://doi.org/10.1007/BF00248757
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00248757