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Biochemical and spectroscopic analysis of UV effects in the marine flagellate Cryptomonas maculata

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Abstract

The effects of solar and artifical ultraviolet radiation on the marine cryptoflagellate, Cryptomonas maculata, were studied. Even after short exposure to UV the accessory photosynthetic pigment phycoerythrin is bleached; likewise the fluorescence undergoes significant changes both in amplitude and in the maximal peak wavelength. In parallel, the photosynthetic oxygen production decreases rapidly during exposure. Gel electrophoresis and FPLC of membrane proteins show a significant decrease in chromoproteins after 2 h UV, which is confirmed by fluorescence excitation and emission spectra of the FPLC fractions.

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Abbreviations

APS:

ammonium persulfate

DCMU:

3-(3′,4′dichlorophenyl)1,1-dimethylurea; Emulphogen, polyoxyethylene 10 tridecyl ether

FPLC:

fast protein liquid chromatography

PMSF:

phenylmethylsulfonyl fluoride

SDS:

sodium dodecylsulfate

SDS PAGE:

sodium dodecylsulfate polyacrylamide gel electrophoresis

TEMED:

NN N′N′tetramethylethylene diamine

UV-A:

wavelength range between 320 nm and 400 nm

UV-B:

wavelength range between 280 nm and 320 nm

References

  • Bornmann JF (1989) Target sites of UV-B radiation in photosynthesis of higher plants. J Photochem Photobiol B: Biol 4:145–158

    Google Scholar 

  • Brown JS (1988) Photosynthetic pigment organization in diatoms (Bacillariophyceae). J Phycol 24:96–102

    Google Scholar 

  • Burns NM, Rosa F (1980) In situ measurements of the settling velocity of organic carbon particles and ten species of phytoplankton. Limnol Oceanogr 25:855–864

    Google Scholar 

  • Dubinsky Z, Falkowski PG, Post AF, Hes UMvan (1987) A system for measuring phytoplankton photosynthesis in a defined light field with an oxygen electrode. J Plankton Res 9:607–612

    Google Scholar 

  • El-Sayed SZ (1988) Fragile life under the ozone hole. Nat Hist 97:72–81

    Google Scholar 

  • Estabrook RW (1967) Mitocondrial respiratory control and the polarographic measurement of ADP:O ratios. In: Estabrook RW, Pullmann ME (eds) Methods in enzymology, vol. X. Academic Press, New York, pp 41–47

    Google Scholar 

  • Görg A, Postel W, Günther S (1988) The current state of two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis 9. 31–546

    Google Scholar 

  • Häder D-P (1986) Effects of solar and artifical UV irradiation on motility and phototaxis in the flagellate, Euglena gracilis. Photochem Photobiol 44:651–656

    Google Scholar 

  • Häder D-P, Häder M (1988a) Ultraviolet-B inhibition of motility in green and dark bleached Euglena gracilis. Curr Microbiol 17:215–220

    Google Scholar 

  • Häder D-P, Häder MA (1988b) Inhibition of motility and phototaxis in the green flagellate, Euglena gracilis, by UV-B radiation. Arch Microbiol 150:20–25

    Google Scholar 

  • Häder D-P, Häder MA (1989a) Effects of solar UV-B irradiation on photomovement and motility in photosynthetic and colorless flagellates. Environ Exp Bot 29:273–282

    Google Scholar 

  • Häder D-P, Häder MA (1989b) Effects of solar and artificial radiation on motility and pigmentation in Cyanophora paradoxa. Arch Microbiol 152:453–457

    Google Scholar 

  • Häder D-P, Häder M (1989c) Effects of solar radiation on photo-orientation, motility and pigmentation in freshwater Cryptomonas. Botanica Acta 102:236–240

    Google Scholar 

  • Häder D-P, Häder M (1990) Effects of UV radiation on motility, photo-orientation and pigmentation in a freshwater Cryptomonas. J Photochem Photobiol B: Biol 5:105–114

    Google Scholar 

  • Häder D-P, Häder M, Liu S-M, Ullrich W (1990a) Effects of solar radiation on photoorientation, motility and pigmentation in a freshwater Peridinium. BioSystems 23:335–343

    Google Scholar 

  • Häder D-P, Liu S-M, Häder M, Ullrich W (1990b) Photoorientation, motility and pigmentation in a freshwater Peridinium affected by ultraviolet radiation. Gen Physiol Biophys 9:361–371

    Google Scholar 

  • Häder D-P, Liu S-M (1990a) Effects of artificial and solar UV-B radiation on the gravitactic orientation of the dinoflagellate, Peridinium gatunense. FEMS Microbiol Ecol 73:331–338

    Google Scholar 

  • Häder D-P, Liu S-M (1990b) Motility and gravitactic orientation of the flagellate, Euglena gracilis, impaired by artificial and solar UV-B radiation. Curr Microbiol 21:161–168

    Google Scholar 

  • Häder D-P, Rhiel E, Wehrmeyer WJ (1987) Phototaxis in the marine flagellate Cryptomonas maculata. J Photochem Photobiol 1:115–122

    Google Scholar 

  • Häder D-P, Rhiel E, Wehrmeyer W (1988) Ecological consequences of photomovement and photobleaching in the marine flagellate Cryptomonas maculata. FEMS Microbiol Ecol 53:9–18

    Google Scholar 

  • Häder D-P, Worrest RC (1991) Effects of enhanced solar ultraviolet radiation on aquatic ecosystems. Photochem Photobiol 53:717–725

    Google Scholar 

  • Houghton RA, Woodwell GM (1989) Global climatic change. Sci Am 260:18–26

    Google Scholar 

  • Jerlov NG (1976) Marine optics. Elsevier, Amsterdam

    Google Scholar 

  • Kamiya R, Miyachi S (1984) Effects of light quality on formation of 5-aminolevulinic acid, phycoerythrin and chlorophyll in Cryptomonas sp. cells collected from the subsurface chlorophyll layer. Plant Cell Physiol 25:831–839

    Google Scholar 

  • Mörschel E, Wehrmeyer W (1975) Cryptomonad biliprotein: phycocyanin-645 from a Chroomonas species. Arch Microbiol 105:153–158

    Google Scholar 

  • Mörschel E, Wehrmeyer W (1977) Multiple forms of phycoerythrin-545 from Cryptomonas maculata. Arch Microbiol 113:83–89

    Google Scholar 

  • Provasoli L, McLaughlin JJ, Droop MR (1957) The development of artificial media for marine algae. Arch Microbiol 25:392–428

    Google Scholar 

  • Raven JA (1991) Responses of aquatic photosynthetic organisms to increased solar UVB. J Photochem Photobiol [B] 9:239–244

    Google Scholar 

  • Renger G, Völker M, Eckert HJ, Fromme R, Hohm-Veit S, Gräber P (1989) On the mechanism of photosystem II deterioration by UV-B irradiation. Photochem Photobiol 49:97–105

    Google Scholar 

  • Rhiel E Krupinska K, Wehremeyer W (1986) Effects of nitrogen starvation on the function and organization of the photosynthetic membranes in Cryptomonas maculata (Cryptophyceae). Planta 169:361–369

    Google Scholar 

  • Rhiel E, Häder D-P, Wehrmeyer WJ (1988a) Photo-orientation in a freshwater Cryptomonas species. J Photochem Photobiol [B] 2:123–132

    Google Scholar 

  • Rhiel E, Häder D-P, Wehrmeyer W (1988b) Diaphototaxis and gravitaxis in a freshwater Cryptomonas. Plant Cell Physiol 29:755–760

    Google Scholar 

  • Watanabe M, Furuya M (1974) Action spectrum of phototaxis in a cryptomonad alga, Cryptomonas sp. Plant Cell Physiol 15:413–420

    Google Scholar 

  • Watanabe M, Miyoshi Y, Furuya M (1976) Phototaxis in Cryptomonas sp. under condition suppressing photosynthesis. Plant Cell Physiol 17:683–690

    Google Scholar 

  • Williamson P, Holligan PM (1990) Ocean productivity and climate change. Trends Ecol Evol 5:299–303

    Google Scholar 

  • Worrest RC (1983) Impact of solar ultraviolet-B radiation (290–320 nm) upon marine microalgae. Physiol Plant 58:428–434

    Google Scholar 

Download references

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Authors and Affiliations

  1. Institut für Botanik und Pharmazeutische Biologie, Friedrich-Alexander Universität, Staudtstrasse 5, W-8520, Erlangen, Federal Republic of Germany

    Ilse Zündorf & Donat-P. Häder

Authors
  1. Ilse Zündorf
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  2. Donat-P. Häder
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Dedicated to the 60th birthday of Professor Dr. W. Wehrmeyer

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Zündorf, I., Häder, DP. Biochemical and spectroscopic analysis of UV effects in the marine flagellate Cryptomonas maculata . Arch. Microbiol. 156, 405–411 (1991). https://doi.org/10.1007/BF00248718

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  • DOI: https://doi.org/10.1007/BF00248718

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