Archives of Microbiology

, Volume 155, Issue 2, pp 183–190 | Cite as

Anaerobic aniline degradation via reductive deamination of 4-aminobenzoyl-CoA in Desulfobacterium anilini

  • Sylvia Schnell
  • Bernhard Schink
Original Papers


The initial reactions involved in anaerobic aniline degradation by the sulfate-reducing Desulfobacterium anilini were studied. Experiments for substrate induction indicated the presence of a common pathway for aniline and 4-aminobenzoate, different from that for degradation of 2-aminobenzoate, 2-hydroxybenzoate, 4-hydroxybenzoate, or phenol. Degradation of aniline by dense cell suspensions depended on CO2 whereas 4-aminobenzoate degradation did not. If acetyl-CoA oxidation was inhibited by cyanide, benzoate accumulated during degradation of aniline or 4-aminobenzoate, indicating an initial carboxylation of aniline to 4-aminobenzoate, and further degradation via benzoate of both substrates. Extracts of alinine or 4-aminobenzoategrown cells activated 4-aminobenzoate to 4-aminobenzoyl-CoA in the presence of CoA, ATP and Mg2+. 4-Aminobenzoyl-CoA-synthetase showed a Km for 4-aminobenzoate lower than 10 μM and an activity of 15.8 nmol · min-1 · mg-1. 4-Aminobenzoyl-CoA was reductively deaminated to benzoyl-CoA by cell extracts in the presence of low-potential electron donors such as titanium citrate or cobalt sepulchrate (2.1 nmol · min-1 · mg-1). Lower activities for the reductive deamination were measured with NADH or NADPH. Reductive deamination was also indicated by benzoate accumulation during 4-aminobenzoate degradation in cell suspensions under sulfate limitation. The results provide evidence that aniline is degraded via carboxylation to 4-aminobenzoate, which is activated to 4-aminobenzoyl-CoA and further metabolized by reductive deamination to benzoyl-CoA.

Key words

Carboxylation 4-Aminobenzoate Benzoate 4-Aminobenzoyl-CoA synthetase 4-Aminobenzoyl-CoA reductase 


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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Sylvia Schnell
    • 1
  • Bernhard Schink
    • 1
  1. 1.Lehrstuhl Mikrobiologie IEberhard-Karls-UniversitätTübingenGermany

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