Abstract
Preincubation of yeast cells in the presence of benzoate or sorbate at an extracellular pH value of 6.8 elicited a set of metabolic effects on sugar metabolism, which became apparent after the subsequent glucose addition. They can be summarized as follows: a) reduced glucose consumption; b) inhibition of glucose- and fructose-phosphorylating activities; c) supression of glucose-triggered peak of hexoses monophosphates; d) substantial reduction of glucose-triggered peak of fructose 2,6-bisphosphate; e) block of catabolite inactivation of fructose-1,6-bisphosphatase and phosphoenolpyruvate carboxykinase, but not of cytoplamic malate dehydrogenase. On the whole this pattern resulted in prevention of glucose-induced switch of metabolism from a gluconeogenetic to a glycolytic state. Our data also show that, unlike former assumptions, intracellular acidification is not likely to mediate the bulk of metabolic effects of benzoate and sorbate, since under our working conditions intracellular pH kept close to neutrality.
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References
Bergmeyer HU (1974) Methods of enzymatic analysis. Verlag Chemie, Weinheim, and Academic Press, New York London
Bisson LF, Fraekel DG (1983) Involvement of kinases in glucose and fructose uptake by Saccharomyces cerevisiae. Proc Natl. Acad Sci USA 80: 1730–1734
Entian K-D, Mecke D (1982) Genetic evidence for a role of hexokinase isozyme PII in carbon catabolite repression in Saccharomyces cerevisiae. J Biol Chem 27: 870–874
Entian K-D, Kopetzki E, Fröhlich K-U, Mecke D (1984) Cloning of hexokinase isoenzyme PI from Saccharomyces cerevisiae: PI transformants confirm the unique role of hexokinase isoenzyme PII for glucose repression in yeasts. Mol Gen Genet 198: 50–54
François J, Van Schaftingen E, Hers H-G (1984) The mechanism by which glucose increases fructose 2,6-bisphosphate concentration in Saccharomyces cerevisiae. A cyclic-AMP-dependent activation of phosphofructokinase 2. Eur J Biochem 145: 187–193
François J, Van Schaftingen E, Hers H-G (1986) Effect of benzoate on the metabolism of fructose 2,6-bisphosphate in yeast. Eur J Biochem 154: 141–145
Funayama S, Gancedo JM, Gancedo C (1980) Turnover of yeast fructose-1,6-bisphosphatase in different metabolic conditions. Eur J Biochem 109: 61–66
Gancedo JM, Gancedo C (1979) Inactivation of gluconeogenetic enzymes in glycolytic mutants of Saccharomyces cerevisiae. Eur J Biochem 101: 455–460
Holzer H (1976) Catabolite inactivation in yeast. Trends Biochem Sci 1: 178–181
Krebs HA, Wiggins D, Stubbs M, Sols A, Bedoya F (1983) Studies on the mechanism of the antifungal action of benzoate. Biochem J 214: 657–663
Lang JM, Cirillo VP (1987) Glucose transport in a kinaseless Saccharomyces cerevisiae mutant. J Bacteriol 169: 2932–2937
Lederer B, Vissers S, Van Schaftingen E, Hers H-G (1981) Fructose 2,6-bisphosphate in yeast. Biochem Biophys Res Commun 103: 1281–1287
Mazòn MJ, Gancedo JM, Gancedo C (1982) Inactivation of yeast fructose-1,6-bisphosphatase. J Biol Chem 257: 1128–1130
Müller D, Holzer H (1981) Regulation of frunctose-1,6-bisphosphatase in yeast by phosphorylation/dephosphorylation. Biochem Biophys Res Commun 103: 926–933
Müller M, Müller H, Holzer H (1981) Immunochemical studies on catabolite inactivation of phosphoenolpyruvate carboxykinase in Saccharomyces cerevisiae. J Biol Chem 256: 723–727
Peña P de la, Barros F, Gascòn S, Ramos S, Lazo PS (1982) The electrochemical proton gradient of Saccharomyces cerevisiae. The role of potassium. Eur J Biochem 123: 447–453
Purwin C, Leidig F, Holzer H (1982) Cyclic AMP-dependent phosphorylation of fructose-1,6-bisphosphatase in yeast. Biochem Biophys Res Commun 107: 1482–1489
Ramos J, Cirillo VP (1989) Role of cyclic-AMP-dependent protein kinase in catabolite inactivation of the glucose and galactose transporters in Saccharomyces cerevisiae. J Bacteriol 171: 3545–3548
Ramos J, Skutnicka K, Cirillo VP (1988) Relationship between low- and high- affinity glucose transport systems of Saccharomyces cerevisiae. J Bacteriol 170: 5375–5377
Rose M, Entian K-D, Hofmann L, Vogel RD, Mecke D (1988) Irreversible inactivation of Saccharomyces cerevisiae fructose-1,6-bisphosphatase independent of protein phosphorylation at Ser11. FEBS Lett 241: 55–59
Tortora P, Birtel M, Lenz AG, Holzer H (1981) Glucose dependent metabolic interconversion of fructose-1,6-bisphosphatase in yeast. Biochem Biophys Res Commun 100: 335–341
Tortora P, Burlini N, Hanozet GM, Guerritore A (1982) Effect of caffeine on glucose-induced inactivation of gluconeogenetic enzymes in Saccharomyces cerevisiae. A possible role of cyclic AMP. Eur J Biochem 126: 617–622
Tortora P, Burlini N, Caspani G, Guerritore A (1984) Studies on glucose-induced inactivation of gluconeogenetic enzymes in adenylate cyclase and cAMP-dependent protein kinase yeast mutants. Eur J Biochem 145: 543–548
Van Schaftingen E, Lederer B, Bartrons R, Hers H-G (1982) A kinetic study of pyrophosphate: fructose-6-phosphate phosphotransferase from potato tubers. Application to a microassay of fructose 2,6-bisphosphate. Eur J Biochem 129: 191–195
Yamashoji S, Hess B (1984a) Yeast 6-phosphofructo-2-kinase. FEBS Lett 172: 51–54
Yamashoji S, Hess B (1984b) Activation of yeast 6-phosphofructose-2-kinase by protein kinase and phosphate. FEBS Lett 178: 253–256
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Burlini, N., Pellegrini, R., Facheris, P. et al. Metabolic effects of benzoate and sorbate in the yeast Saccharomyces cerevisiae at neutral pH. Arch. Microbiol. 159, 220–224 (1993). https://doi.org/10.1007/BF00248475
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DOI: https://doi.org/10.1007/BF00248475