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Subunits of tetrameric α-amylase inhibitors of Hordeum chilense are encoded by genes located in chromosomes 4Hch and 7Hch

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Summary

Three proteins (components 1, 2, and 4) of the non-prolamin, 70% ethanol soluble fraction from the endosperm of Hordeum chilense have been identified as putative subunits of the tetrameric inhibitors active against insect α-amylases. In experiments carried out with the synthetic alloploid Tritordeum (H. chilense x Triticum turgidum conv. durum), previously described proteins from T. turgidum, designated CM2, CM3 and CM 16, have been also identified as subunits of α-amylase inhibitors. Genes for components 1 and 4 of H. chilense have been located in chromosomes 4Hch and 7Hch, based on the analysis of H. chilense-T.turgidum addition lines. Subunits of the inhibitors from wheat and from cultivated barley had been previously assigned to chromosomes of the same homoeology groups.

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References

  • Aragoncillo C, Rodriguez-Loperena MA, Carbonero P, García-Olmedo F (1975) Chromosomal control of non-gliadin proteins from the ethanol extract of wheat endosperm. Theor Appl Genet 45:322–326

    Google Scholar 

  • Barber D, Sanchez-Monge R, Mendez E, Lazaro A, García-Olmedo F, Salcedo G (1986a) New α-amylase and trypsin inhibitors among the CM proteins of barley (Hordeum vulgare L.). Biochim Biophys Acta 869:115–118

    Google Scholar 

  • Barber D, Sanchez-Monge R, García-Olmedo F, Salcedo G, Mendez E (1986b) Evolutionary implications of sequential homologies among members of the trypsin/α-amylase inhibitor family (CM proteins) in wheat and barley. Biochim Biophys Acta 873:147–151

    Google Scholar 

  • Buonocore V, De Biasi M-G, Giardina P, Poerio E, Silano V (1985) Purification and properties of an α-amylase tetrameric inhibitor from wheat kernel. Biochim Biophys Acta 831:40–48

    Google Scholar 

  • Fernandez JA (1986) Caracteristicas geneticas y citogeneticas de Hordeum chilense y de su anfiploide x Triticum turgidum conv. durum. PhD Thesis, University of Alcala de Henares, Alcala de Henares, Madrid

    Google Scholar 

  • Fernandez JA, Jouve N (1984) Giemsa C-banding of the chromosomes of Hordeum chilense and its amphiploid x Triticum turgidum conv. durum. Z Pflanzenzücht 93:212–221

    Google Scholar 

  • Fernandez JA, Jouve N (1987a) Chromosomal location of structural genes controlling isozymes in Hordeum chilense. 1. 6-Phosfogluconate dehydrogenase and malate dehydrogenase. Theor Appl Genet 73:433–439

    Google Scholar 

  • Fernandez JA, Jouve N (1987b) Chromosomal location of structural genes controlling isozymes in Hordeum chilense. 3. Esterases, glutamate oxaloacetate transaminase and phosphoglucomutase. Theor Appl Genet 73:690–698

    Google Scholar 

  • Fernandez JA, Jouve N (1987c) The addition of Hordeum chilense chromosomes to Triticum turgidum conv. durum. Biochemical, karyological and morphological characterization. Euphytica (in press)

  • Fra-Mon P, Salcedo G, Aragoncillo C, García-Olmedo F (1984) Chromosomal assignment of genes encoding salt-soluble proteins (albumins and globulins) in wheat and related species. Theor Appl Genet 69:167–172

    Google Scholar 

  • García-Olmedo F, Carbonero P, Jones BL (1982) Chromosomal locations of genes that control wheat endosperm proteins. In: Pomeranz Y (ed) Advances in cereal science and technology, vol V. AACC, St Paul Minn, pp 1–47

    Google Scholar 

  • Love A (1982) Generic evolution of the wheatgrasses. Biol Zentralbl 101:199–212

    Google Scholar 

  • Martin A, Sanchez-Monge Laguna E (1982) Cytology and morphology of the amphiploid Hordeum chilense x Triticum turgidum conv. durum. Euphytica 31:261–267

    Google Scholar 

  • Miller TE, Reader SM, Ainsworth CC (1985) A chromosome of Hordeum chilense homoeologous to group 7 of wheat. Can J Genet Cytol 27:101–104

    Google Scholar 

  • Molina-Cano JL, Fra-Mon P, Salcedo G, Aragoncillo C, Roca de Togores F, García-Olmedo F (1987) Morocco as a possible domestiation center for barley: biochemical and agromorphological evidence. Theor Apl Genet 73:531–536

    Google Scholar 

  • Padilla JA, Martin A (1983) New hybrids between Hordeum chilense and tetraploid wheats. Cereal Res Commun 11:5–7

    Google Scholar 

  • Rodriguez-Loperena MA, Aragoncillo C, Carbonero P, García-Olmedo F (1975) Heterogeneity of wheat endosperm proteolipids (CM proteins). Phytochemistry 14:1219–1223

    Google Scholar 

  • Salcedo G, Sanchez-Monge R, Argamenteria A, Aragoncillo C (1980) The A-hordeins as a group of salt soluble hydrophobic proteins. Plant Sci Lett 19:109–119

    Google Scholar 

  • Salcedo G, Sanchez-Monge R, Aragoncillo C (1982) The isolation and characterization of low molecular weight hydrophobic salt-soluble proteins from barley. J Exp Bot 33:1325–1331

    Google Scholar 

  • Salcedo G, Fra-Mon P, Molina-Cano JL, Aragoncillo C, García-Olmedo F (1984) Genetics of CM-proteins (A-hordeins) in barley. Theor Appl Genet 68:53–59

    Google Scholar 

  • Sanchez-Monge R, Barber D, Mendez E, García-Olmedo F, Salcedo G (1986a) Genes encoding α-amylase inhibitors are located in the short arms of chromosomes 3B, 3D and 6D of wheat (Triticum aestivum L.). Theor Appl Genet 72:108–113

    Google Scholar 

  • Sanchez-Monge R, Gomez L, García-Olmedo F, Salcedo G (1986b) A tetrameric inhibitor of insect α-amylase from barley. FEBS Lett 207:105–109

    Google Scholar 

  • Shewry PR, Miflin BJ (1985) Seed storage proteins of economically important cereals. In: Pomeranz Y (ed) Advances in cereal science and technology, vol III. AACC, St Paul Minn, pp 1–83

    Google Scholar 

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Communicated by G. Wenzel

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Sanchez-Monge, R., Fernandez, J.A. & Salcedo, G. Subunits of tetrameric α-amylase inhibitors of Hordeum chilense are encoded by genes located in chromosomes 4Hch and 7Hch . Theoret. Appl. Genetics 74, 811–816 (1987). https://doi.org/10.1007/BF00247561

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