Skip to main content
SpringerLink
Log in

Nodulation and nitrogen fixation mutants of pea, Pisum sativum

  • Originals
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Summary

The 36 mutants which did not nodulate and 24 mutants which formed inefficient nodules with no or very low acetylene reduction activity were isolated among 86,000 M2-seedlings of ‘Finale’ pea, Pisum sativum L., after treatment with chemical mutagens. One mutant was found for approximately every 50 chlorophyll mutants. Most mutations were induced by ethyl methanesulfonate; some by diethyl sulfate, ethyl nitrosourea and acidified sodium azide. Putative mutants were selected as nitrogen deficient plants, yellowing from the bottom and up, when M2 seedlings were grown in sand with a Rhizobium mixture and PK fertilizer. The mutants were verified in the M3 generation by acetylene reduction assay on intact plants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bond DA (1984) Yield stability of Faba beans and peas in EEC joint trials 1980–1982. In: Hebblethwaite PD, Dawkins TCK, Heath MC, Lockwood G (eds) Vicia faba: agronomy, physiology and breeding. Nijhoff/Junk, The Hague, pp 177–184

    Google Scholar 

  • Caldwell BE, Vest HG (1977) Genetic aspects of nodulation and dinitrogen fixation by legumes: the macrosymbiont. In: Hardy RWF, Silver WS (eds) A treatise on dinitrogen fixation, Sect III. Biology. Wiley, New York, pp 557–576

    Google Scholar 

  • Carroll BJ, McNeil D, Gresshoff PM (1985) Isolation and properties of soybean (Glycine max (L.) Merr.) mutants that nodulate in the presence of high nitrate concentrations. Proc Natl Acad Sci USA 82:4162–4166

    Google Scholar 

  • Davis TM, Foster KW, Phillips DA (1985) Nodulation mutants in chickpea. Crop Sci 25:345–348

    Google Scholar 

  • Duc G, Picard J, Larambergue H, Mariotti A, Amarger N (1985) Exploration in Vicia faba species of genetic variability for characters associated with nitrogen fixation. In: OECD. Analysis of the plant genes involved in the Legume-Rhizobium symbiosis, OECD, Paris, pp 33–38

    Google Scholar 

  • Gresshoff PM, Day DA, Delves AC, Mathews AP, Olsson JE, Price GD, Schuller KA, Carroll BJ (1985) Plant host genetics of nodulation and symbiotic nitrogen fixation in pea and soybean. In: Evans HJ, Bottomley PJ, Newton WE (eds) Nitrogen fixation research progress. Martinus Nijhoff, Dordrecht, pp 19–25

    Google Scholar 

  • IAEA (1977) Manual on mutation breeding, 2nd edn. IAEA, Vienna

    Google Scholar 

  • Jacobsen, E (1984) Modification of symbiotic interaction of pea (Pisum sativum L.) and Rhizobium leguminosarum by induced mutations. Plant Soil 82:427–438

    Google Scholar 

  • Jacobsen E, Feenstra WJ (1984) A new pea mutant with efficient nodulation in the presence of nitrate. Plant Sci Lett 33:337–344

    Google Scholar 

  • Jacobsen E, Postma JG, Nijdam H, Feenstra WJ (1985) Genetical and grafting experiments with pea mutants in studies on symbiosis. In: Evans HJ, Bottomley PJ, Newton WE (eds) Nitrogen fixation research progress. Martinus Nijhoff, Dordrecht, p 43

    Google Scholar 

  • Kneen BE, LaRue TA (1984) Nodulation resistant mutant of Pisum sativum (L.). J Hered 75:238–240

    Google Scholar 

  • Kneen BE, LaRue TA (1985) Induced mutants of Pisum sativum defective in nodulation. In: Evans HJ, Bottomley PJ, Newton WE (eds) Nitrogen fixation research progress. Martinus Nijhoff, Dordrecht, p 44

    Google Scholar 

  • LaRue TAG, Kurz WGW (1973) Estimation of nitrogenase in intact legumes. Can J Microbiol 19:304–305

    Google Scholar 

  • Lie TA (1984) Host genes in Pisum sativum L. conferring resistance to European Rhizobium leguminosarum strains. Plant Soil 82:415–425

    Google Scholar 

  • Mathews A, Carroll BJ, McNeil D, Gresshoff PM (1985) Isolation and characterization of non-nodulating mutants of soybeans. In: Evans HJ, Bottomley PJ, Newton WE (eds) Nitrogen fixation research progress. Martinus Nijhoff, Dordrecht, p 45

    Google Scholar 

  • Messager A (1985) Selection of pea mutants for nodulation and nitrogen fixation. In: OECD. Analysis of the plant genes involved in the Legume-Rhizobium symbiosis. OECD, Paris, pp 52–60

    Google Scholar 

  • Nutman PS (1984) Improving nitrogen fixation in legumes by plant breeding; the relevance of host selection experiments in red clover (Trifolium pratense L.) and subterranean clover (T. subterraneum L.). Plant Soil 82:285–301

    Google Scholar 

  • Ohlendorf H (1983) Untersuchungen zur Vererbung der Resistenz von Pisum sativum gegen Rhizobium leguminosarum Stamm 311d. Z Pflanzenzücht 91:13–24

    Google Scholar 

  • Peterson MA, Barnes DK (1981) Inheritance of ineffective nodulation and non-nodulation traits in alfalfa. Crop Sci 21:611–616

    Google Scholar 

  • Wilson DO, Reisenauer HM (1963) Cobalt requirement of symbolically grown alfalfa. Plant Soil 19:364–373

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Agricultural Research Department, Risø National Laboratory, DK-4000, Roskilde, Denmark

    K. C. Engvild

Authors
  1. K. C. Engvild
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by P.M.A. Tigerstedt

Rights and permissions

Reprints and permissions

About this article

Cite this article

Engvild, K.C. Nodulation and nitrogen fixation mutants of pea, Pisum sativum . Theoret. Appl. Genetics 74, 711–713 (1987). https://doi.org/10.1007/BF00247546

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00247546

Key words

Search

Navigation