Summary
From two lines of Medicago sativa characterized by a high regeneration capability, calli resistant to culture filtrate of Fusarium oxysporum f. sp. medicaginis have been selected. In these calli regeneration capability was greatly reduced and only one plant per callus was recovered. Regenerated plants have been evaluated for resistance to culture filtrate and for in vivo resistance to the pathogen. Three plants out of eight were resistant to the fungus and a high correlation between resistance to culture filtrate and in vivo resistance was observed.
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Arcioni S, Mariotti D, Pezzotti M (1985) Hedysarum coronarium L. In vitro conditions for plant regeneration from protoplasts and callus of various explants. J Plant Physiol 121:141–148
Buiatti M, Scala A, Bettini P, Nascari G, Morpurgo R, Bogani P, Pellegrini G, Gimelli F, Venturo R (1985) Correlations between in vivo resistance to Fusarium and in vitro response to fungal elicitor and toxic substances in carnation. Theor Appl Genet 70:42–47
Collins RP, Scheffer RP (1958) Respiratory responses and systemic effects in Fusarium infected tomato plants. Phytopathology 48:349–355
Elgin JH (1981) Use of strain crosses in breeding multiple pest resistance alfalfa. Report, 27th Alfalfa Improvement Conference, 8–10 July, 1980. University of Wisconsin, Madison, p 58
Foroughi-Wehr B, Friedt W, Schuchmann R, Kohler F, Wenzel G (1986) In vitro selection for resistance. In: Semal J (ed) Somaclonal variations and crop improvement. Martinus Nijhoff, pp 35–44
Frosheiser FI, Barnes DK (1978) Field reaction of artificially inoculated alfalfa populations to the Fusarium and bacterial wilt pathogens alone and in combination. Phytopathology 68:943–946
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension culture of soybean root cells. Exp Cell Res 50:151–158
Gengenbach BG, Green CE, Donovan CN (1978) Inheritance of selected pathotoxin resistance in maize plants regenerated from cell cultures. Proc Natl Acad Sci USA 74:5113–5117
Gondran J (1986) Critères de sélection pour la résistence aux maladies des luzernes cultivées dans les regions mediterranéennes en zones irriguées et non irriguées. CR Miss Ital INRA 1986
Graham JH, Frosheiser FI, Stuteville DL, Erwin DC (1979) Fusarium wilt. In: A compendium of alfalfa diseases. Am Phytopathol Soc Monogr, p 36
Hartmann CL, McCoy TJ, Knous TR (1984) Selection of alfalfa (Medicago sativa) cell lines and regeneration of plants resistant to the toxin(s) produced of Fusarium oxysporum f. sp. medicaginis. Plant Sci Lett 34:183–194
Heinz DJ, Krishnamurthi M, Nickell LG, Maretzki A (1977) Cell, tissue and organ culture in sugarcane improvement. In: Reinert J, Bajaj YPS (eds) Plant tissue and cell culture. Springer, New York, pp 3–17
Hijano EH, Barnes DK, Frosheiser FI (1983) Inheritance of resistance to Fusarium wilt in alfalfa. Crop Sci 23:31–34
Larkin PJ, Scowcroft WR (1981) Somaclonal variation: a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60:197–214
Latunde-Dada AO, Lucas JA (1983) Somaclonal variation and reaction to Verticillium wilt in Medicago sativa L. plants regenerated from protoplast. Plant Sci Lett 32:205–211
Ling DH, Vidhyaseharam D, Borromeo ES, Zapata FJ, Mew TW (1985) In vitro screening of rice germplasm for resistance to brown spot disease using phytotoxin. Theor Appl Genet 71:133–135
Lupotto E (1983) Propagation of an embryogenetic culture of Medicago sativa L. Z Pflanzenphysiol 3:95–104
Matern U, Strobel G, Shepard J (1978) Reaction to phytotoxin in a potato population derived from mesophyll protoplasts. Proc Natl Acad Sci USA 75:4935–4939
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 15:473–497
Naef-Roth S (1972) Production and bioassay of phytotoxins. In: Wood RKS, Ballio A, Graniti A (eds) Phytotoxin in plant diseases. Academic Press, London New York, pp 49–69
Pezzotti M, Arcioni S, Mariotti D (1984) Plant regeneration from mesophyll, root and cell suspension protoplasts of Medicago sativa cv. Adriana. Genet Agrar 38:195–208
Raynal G (1986) Les maladies de la luzerne en Europe. In: Guy P, Massenot M (eds) Le production de fourrage et de semences de luzerne en Europe: maladies, ravageurs et variétés. Compte-rendu des groupes de travail on Medicago sativa. Eucarpia. INRA, Paris, pp 7–14
Sacristan MD (1982) Resistance response to Phoma lingam of plant regenerated from selected cells and embryogenic cultures of haploid Brassica napus. Theor Appl Genet 61:193–200
Samaddar KR, Scheffer RP (1968) Effects of the specific toxin in Helminthosporium victoriae on host cell membranes. Plant Physiol 43:21–28
Scala A, Bettini P, Buiatti M, Bogani P, Pellegrini G, Tognoni F (1984) In vitro analysis of the tomato Fusarium oxysporum system and selection experiments. In: Novak FJ, Havel L, Dolezel J (eds) Proc Int Symp Plant Tissue and Cell Culture, application to crop improvment, 24–29 Sept, Olomuc, Czechoslovakia, p 361
Shepard JF, Sidney D, Shahin E (1980) Potato protoplast in crop improvement. Science 208:17–24
Thanutong P, Furusawa L, Yamamoto M (1983) Resistant tobacco plants from protoplast derived callus selected for their resistance to Pseudomonas and Alternaria toxins. Theor Appl Genet 66:209–215
Wheeler H, Black HS (1963) Effects of Helminthosporium victoriae and victorin upon permeability. Am J Bot 50:686–693
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Communicated by F. Salamini
Research work supported by C.N.R., Italy. Special grant I.P.R.A. Subproject 1, paper no. 1468
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Arcioni, S., Pezzotti, M. & Damiani, F. In vitro selection of alfalfa plants resistant to Fusarium oxysporum f. sp. medicaginis . Theoret. Appl. Genetics 74, 700–705 (1987). https://doi.org/10.1007/BF00247544
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DOI: https://doi.org/10.1007/BF00247544