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Theoretical and Applied Genetics

, Volume 74, Issue 6, pp 687–692 | Cite as

Chromosome studies of progenies of tetraploid female rainbow trout

  • D. Chourrout
  • I. Nakayama
Originals

Summary

Nine induced tetraploid females were artificially inseminated by UV-irradiated sperm collected from diploid males, in order to induce the gynogenetic development of their ova. Most of the resulting embryos were diploid (or minor aneuploids). Several gynogenetic tetraploids, likely to issue from unreduced ova, were also detected in these progenies. The same females fertilized by normal sperm of diploid males gave a majority of triploids and several pentaploids, while the fertilization by normal sperm of tetraploid males gave rise to a majority of tetraploids and one hexaploid. The same crosses, after the eggs had been heat-shocked to double the maternal genetic contribution, yielded about three-quarters pentaploids and one quarter haploids (normal sperm of diploids), or three-quarters hexaploids and one quarter diploids (normal sperm of tetraploids). These haploids and diploids are likely to result from androgenesis.

Key words

Fish Salmonids Polyploidy Gynogenesis 

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References

  1. Allendorf FW, Seeb JE, Knudsen KL, Thorgaard GH, Leary RF (1986) Gene-centromere mapping of 25 loci in rainbow trout. J Hered 77:307–312Google Scholar
  2. Beetschen JC (1962) Sur la descendance de femelles tetraploides croisées avec des mâles diploides, chez l'amphibien urodèle, Pleurodeles waltlii. CR Acad Sci, Ser D 255:3068–3070Google Scholar
  3. Bidwell CA, Chrisman CL, Libey GS (1985) Polyploidy induced by heat shock in channel catfish. Aquaculture 51:25–32Google Scholar
  4. Bingham ET (1980) Maximizing heterozygosity in autopolyploids. In: Lewis WH (ed) Polyploidy, biological relevance, part IV. Plenum Press, New York, pp 471–490Google Scholar
  5. Chourrout D (1984) Pressure-induced retention of second polar body and suppression of first cleavage in rainbow trout: production of all-triploids, all-tetraploids, heterozygous and homozygous diploid gynogenetics. Aquaculture 36:111–126Google Scholar
  6. Chourrout D (1986) Techniques of chromosome manipulation in rainbow trout: a new evaluation with karyology. Theor Appl Genet 72:627–632Google Scholar
  7. Chourrout D, Happe A (1986) Improved methods of chromosome preparation in rainbow trout, Salmo gairdneri. Aquaculture 52:255–261Google Scholar
  8. Chourrout D, Chevassus B, Krieg F, Happe A, Burger G, Renard P (1986) Production of second generation triploid and tetraploid rainbow trout by mating tetraploid males and diploid females. Theor Appl Genet 72:193–206Google Scholar
  9. Dawson GWP (1962) An introduction to the cytogenetics of polyploids. Blackwell, OxfordGoogle Scholar
  10. Dewey DR (1980) Some applications and misapplications of induced polyploidy to plant breeding. In: Lewis WH (ed) Polyploidy, biological relevance, part IV. Plenun Press, New York, pp 445–470Google Scholar
  11. Diter A, Guyomard R, Chourrout D (1987) Gene segregation in induced tetraploid rainbow trout (Salmo gairdneri Richardson): genetic evidence of preferential pairing of homologous chromosomes. Genome (submitted)Google Scholar
  12. Ellerström S, Sjödin J (1974) Studies on the use of induced autopolyploidy in the breeding of red clover. 3. Frequency and behavior of aneuploids in a tetraploid clover Ley. Z Pflanzenzücht 71:253–263Google Scholar
  13. Fankhauser G, Humphrey RR (1950) Chromosome number and development of progeny of triploid axolotl females mated with diploid males. J Exp Zool 115:207–249Google Scholar
  14. Fankhauser G, Humphrey RR (1959) The origin of spontaneous heteroploids in the progeny of diploid, triploid and tetraploid axolotl females. J Exp Zool 142:379–422Google Scholar
  15. Guyomard R (1984) High level of residual heterozygosity in gynogenetic rainbow trout, Salmo gairdneri Richardson. Theor Appl Genet 67:307–316Google Scholar
  16. Humphrey RR, Fankhauser G (1949) Three generations of polyploids in ambystomid salamanders. J Hered 40:7–12Google Scholar
  17. Kihara H (1951) Triploid watermelons. Proc Am Soc Sci 58:217–230Google Scholar
  18. Myers JM (1985) Tetraploid induction in Oreochromis species. In: 2nd Int Symp Genet Aquacult, Davis Calif (Abstr)Google Scholar
  19. Nishioka M, Ueda H (1983) Studies on polyploidy in Japanese treefrogs. Sci Rep Lab Amphib Biol Hiroshima Univ 6:207–252Google Scholar
  20. Parsons JE, Thorgaard GH (1985) Production of androgenetic diploid rainbow trout. J Hered 76:177–181PubMedGoogle Scholar
  21. Sakharov VV, Kuvarin VV (1970) Aneuploidy in tetraploid rye. Genetika 6:17–22Google Scholar
  22. Thompson D, Scott AP (1984) An analysis of recombination data in gynogenetic diploid rainbow trout. Heredity 53:411–452Google Scholar
  23. Thorgaard GH, Gall GAE (1979) Adult triploids in a rainhow trout family. Genetics 93:961–973Google Scholar
  24. Thorgaard GH, Allendorf FW, Knudsen KL (1983) Gene-centromere mapping in rainbow trout: high interference over long map distances. Genetics 103:771–783Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • D. Chourrout
    • 1
  • I. Nakayama
    • 2
  1. 1.Laboratoire de Génétique des PoissonsINRAJouy-en-JosasFrance
  2. 2.Department of Environmental ScienceHokkaïdo UniversitySapporoJapan

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