Summary
Enzymic conversion of nicotinamide-adenine-dinucleotide (NADP) to Acetylpyridine-adenine-dinucleotidephosphate (APADP) by the transglycosidase activity of brain NAD(P)-nucleosidase was studied and isolation and some properties of these acetylpyridine analogues of NADP are described.
Without reducing the transglycosidase activity, the hydrolysis of NADP is inhibited by its own cleavage-product nicotinamide. Therefore in presence of a high excess of 3-Acetylpyridine or 4-Acetylpyridine, the antimetabolites can be transmitted to 2-Phospho-adenosine-diphosphateribose (ARPPR), forming the analogues of NADP.
The hydrolytic activity of the nucleosidase split 3-APADP faster than NADP and 4-APADP. Following application of 80–100 mg/kg 3-Acetylpyridine 40% of the total NADP of the brain was found as 3-APADP.
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Abbreviations
- NAD:
-
Nicotinamid-adenin-dinucleotid (DPN)
- NADP:
-
Nicotinamid-adenin-dinucleotidphosphat (TPN)
- 3-APAD:
-
3-Acetylpyridin-adenin-dinucleotid
- 3-APADP:
-
3-Acetylpyridin-adenin-dinucleotidphosphat
- 4-APADP:
-
4-Acetylpyridin-adenin-dinucleotidphosphat
- 2′-5′-ADP:
-
Adenosin-2′-5′-diphosphat
- ARPPR:
-
2′-Phosphoadenosindiphosphatribose
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Brunnemann, A., Coper, H. & Herken, H. Biosynthese von 3-Acetylpyridin-adenin-dinucleotidphosphat (3-APADP) aus Nicotinamid-adenin-dinucleotidphosphat (NADP). Naunyn - Schmiedebergs Arch 245, 541–550 (1963). https://doi.org/10.1007/BF00245797
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DOI: https://doi.org/10.1007/BF00245797