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D-Gluconate is an alternative growth substrate for cultivation of Schizosaccharomyces pombe mutants

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Abstract

Schizosaccharomyces pombe cells grow on d-gluconate as the sole carbon and energy source. d-Gluconate is taken up in symport with protons by a specific symporter, ΔpH being the sole driving force. d-Gluconate uptake is independent of the sugar transporting system (e.g. for d-glucose) and of Δψ. The carrier is expressed constitutively, and its activity is not subject to glucose repression. Hence, d-gluconate is a suitable carbon and energy source for growth, when d-glucose or other hexoses have to be eliminated e.g. for selection of mutants deficient in hexose transport.

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Abbreviations

2-DG:

2-deoxy-d-glucose

CCCP:

carbonylcyanide m-chlorophenylhydrazone

ΔpH:

pH-gradient

Δψ:

electrical potential difference across the plasma membrane

SD:

standard deviation

SEM:

standard error of the mean

TPP+ :

tetraphenylphosphonium

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Authors and Affiliations

  1. Botanisches Institut der Universität, Kirschallee 1, W-5300, Bonn, Germany

    Michael Hoever, Brigitta Milbradt & Milan Höfer

Authors
  1. Michael Hoever
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  2. Brigitta Milbradt
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  3. Milan Höfer
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Hoever, M., Milbradt, B. & Höfer, M. D-Gluconate is an alternative growth substrate for cultivation of Schizosaccharomyces pombe mutants. Arch. Microbiol. 157, 191–193 (1992). https://doi.org/10.1007/BF00245289

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  • DOI: https://doi.org/10.1007/BF00245289

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