Abstract
Pediococcus halophilus possesses phosphoenolpyruvate:mannose phosphotransferase system (man:PTS) as a main glucose transporter. A man:PTS defective (man:PTSd) strain X-160 could, however, utilize glucose. A possible glucose-transport mechanism other than PTS was studied with the strain X-160 and its derivative, man:PTSd phosphofructokinase defective (PFK−) strain M-13. Glucose uptake by X-160 at pH 5.5 was inhibited by any of carbonylcyanide m-chlorophenylhydrazone, nigericin, N,N′-dicyclohexylcarbodiimide, or iodoacetic acid. The double mutant M-13 could still transport glucose and accumulated intracellularly a large amount of hexose-phosphates (ca. 8 mM glucose 6-phosphate and ca. 2 mM fructose 6-phosphate). Protonophores also inhibited the glucose transport at pH 5.5, as determined by the amounts of accumulated hexose-phosphates (< 4 mM). These showed involvement of proton motive force (ΔP) in the non-PTS glucose transport. It was concluded that the non-PTS glucose transporter operated in concert with hexokinase or glucokinase for the metabolism of glucose in the man:PTSd strain.
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Abbreviations
- BM:
-
basal medium
- BM-G:
-
basal medium containing glucose
- CM:
-
complex medium
- man:PTS:
-
phosphoenolpyruvate:mannose phosphotransferase system
- CCCP:
-
carbonylcyanide m-chlorophenylhydrazone
- DCCD:
-
N,N′-dicyclohexyl carbodiimide
- ΔP:
-
proton motive force
- ΔpH:
-
transmembrane pH gradient
- Δφ:
-
transmembrane electrical potential difference
- MNNG:
-
N-methyl-N′-nitro-N-nitrosoguanidine
- PIPES:
-
piperazine-N,N′-bis(-ethanesulfonic acid)
- MES:
-
4-morpholineethanesulfonic acid
- G-6-P:
-
glucose 6-phosphate
- F-6-P:
-
fructose 6-phosphate
- FDP:
-
fructose 1,6-bisphosphate
- EMP:
-
Embden-Meyerhof-Parnas pathway
- PFK:
-
phosphofructokinase
- GK:
-
glucokinase
- HK:
-
hexokinase
- IAA:
-
iodoacetic acid
- IIman :
-
enzyme II component of man:PTS
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Abe, K., Uchida, K. Non-PTS uptake and subsequent metabolism of glucose in Pediococcus halophilus as demonstrated with a double mutant defective in phosphoenolpyruvate:mannose phosphotransferase system and in phosphofructokinase. Arch. Microbiol. 153, 537–540 (1990). https://doi.org/10.1007/BF00245262
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DOI: https://doi.org/10.1007/BF00245262