Archives of Microbiology

, Volume 154, Issue 5, pp 489–495 | Cite as

Catabolism of 3-hydroxybenzoate by the gentisate pathway in Klebsiella pneumoniae M5a1

  • David C. N. Jones
  • Ronald A. Cooper
Original Papers


Growth of Klebsiella pneumoniae M5a1 on 3-hydroxybenzoate leads to the induction of 3-hydroxybenzoate monooxygenase, 2,5-dihydroxybenzoate dioxygenase, maleylpyruvate isomerase and fumarylpyruvate hydrolase. Growth in the presence of 2,5-dihydroxybenzoate also induces all of these enzymes including the 3-hydroxybenzoate monooxygenase which is not required for 2,5-dihydroxybenzoate catabolism. Mutants defective in 3-hydroxybenzoate monooxygenase fail to grow on 3-hydroxybenzoate but grow normally on 2,5-dihydroxybenzoate. Mutants lacking maleylpyruvate isomerase fail to grow on 3-hydroxybenzoate and 2,5-dihydroxybenzoate. Both kinds of mutants grow normally on 3,4-dihydroxybenzoate. Mutants defective in maleylpyruvate isomerase accumulate maleylpyruvate when exposed to 3-hydroxybenzoate and growth is inhibited. Secondary mutants that have additionally lost 3-hydroxybenzoate monooxygenase are no longer inhibited by the presence of 3-hydroxybenzoate. The 3-hydroxybenzoate monooxygenase gene (mhbM) and the maleylpyruvate isomerase gene (mhbI) are 100% co-transducible by P1 phage.

Key words

Klebsiella pneumoniae M5a1 3-Hydroxybenzoate degradation Gentisate pathway 3-Hydroxybenzoate monooxygenase mutants Maleylpyruvate isomerase mutants 


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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • David C. N. Jones
    • 1
  • Ronald A. Cooper
    • 1
  1. 1.Department of BiochemistryUniversity of LeicesterLeicesterUK

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