Polyphosphate glucokinase and ATP glucokinase activities in Mycococcus coralloides D
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Polyphosphate glucokinase and ATP glucokinase were detected in cell-free extracts of Myxococcus coralloides strain D, but pyrophosphate glucokinase was not detected. Both glucokinase activities were separated by chromatography. The approximate molecular weight is 61 000 for polyphosphate glucokinase and 47 000 for ATP glucokinase. Substrate specificity and pH optimum was studied in the polyphosphate glucokinase. Polyphosphate and ATP glucokinase activities were verified by 13C Nuclear Magnetic Resonance.
Key words13C-NMR Glucokinase Polyphosphate Myxococcus
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