Abstract
Desulfotomaculum thermobenzoicum strain TSB (DSM 6193) was found to utilize some methoxylated benzoates as carbon and energy source with or without sulfate. 3- or 4-Methoxybenzoate, vanillate (4-hydroxy-3-methoxybenzoate), syringate (3,5-dimethoxy-4-hydroxybenzoate) and 3,4,5-trimethoxybenzoate were converted to corresponding hydroxybenzoates. However, neither 2-methoxybenzoate nor 2,6-dimethoxybenzoate was utilized. The organism grew acetogenically on each of the methoxylated benzoates in the absence of sulfate.
3,4-Dihydroxy-5-methoxybenzoate was detected during conversion of syringate, and syringate and 3,4-dihydroxy-5-methoxybenzoate were detected during conversion of 3,4,5-trimethoxybenzoate as intermediates.
These findings indicate that 4-methoxyl-group is most readily cleaved, whereas 2-methoxyl-group is not utilized by the organism.
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References
Bache R, Pfennig N (1981) Selective isolation of Acetobacterium woodii on methoxylated aromatic acids and determination of growth yields. Arch Microbiol 130: 255–261
DeWeerd KA, Saxena A, Nagle DPJr, Suflita JM (1988) Metabolism of the 18O-methoxy substituent of 3-methoxybenzoic acid and other unlabeled methoxybenzoic acids by anaerobic bacteria. Appl Environ Microbiol 54: 1237–1242
Healy JBJr, Young LY (1979) Anaerobic biodegradation of eleven aromatic compounds to methane. Appl Environ Microbiol 38: 84–89
Kaiser J-P, Hanselmann KW (1982) Fermentative metabolism of substituted monoaromatic compounds by a bacterial community from anaerobic sediments. Arch Microbiol 133: 185–194
Klemps R, Cypionka H, Widdel F, Pfennig N (1985) Growth with hydrogen, and further physiological characteristics of Desulfotomaculum species. Arch Microbiol 143: 203–208
Krumholz LR, Bryant MP (1986) Syntrophococcus sucromutans sp. nov., gen. nov. uses carbohydrates as electron donors and formate, methoxymonobenzenoids or Methanobrevibacter as electron acceptor systems. Arch Microbiol 143: 313–318
Miller JDA, Neumann PM (1970) Malate dismutation by Desulfovibrio. Arch Microbiol 71: 214–219
Miller JDA, Wakerley DS (1966) Growth of sulphate-reducing bacteria by fumarate dismutation. J Gen Microbiol 43: 101–107
Postgate JR (1952) Growth of sulphate-reducing bacteria in sulphate-free media. Research 5: 189–190
Spormann AM and Thauer RK (1988) Anaerobic acetate oxidation to CO2 by Desulfotomaculum acetoxidans. Demonstration of enzymes required for operation of an oxidative acetyl-CoA/carbon monoxide dehydrogenase pathway. Arch Microbiol 150: 374–380
Tasaki M, Kamagata Y, Nakamura K, Mikami E (1991) Isolation and characterization of a thermophilic benzoate-degrading, sulfate-reducing bacterium, Desulfotomaculum thermobenzoicum sp. nov. Arch Microbiol 155: 348–352
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Tasaki, M., Kamagata, Y., Nakamura, K. et al. Utilization of methoxylated benzoates and formation of intermediates by Desulfotomaculum thermobenzoicum in the presence or absence of sulfate. Arch. Microbiol. 157, 209–212 (1992). https://doi.org/10.1007/BF00245151
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DOI: https://doi.org/10.1007/BF00245151